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mouse monoclonal anti-brafv600e antibody, clone ve1  (Spring Bioscience)

 
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    Structured Review

    Spring Bioscience mouse monoclonal anti-brafv600e antibody, clone ve1
    Mouse Monoclonal Anti Brafv600e Antibody, Clone Ve1, supplied by Spring Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti-brafv600e+(ve1)+mouse+monoclonal+antibody/braf+v600e+antibody/10__1097_slash_pgp__0000000000000933-45-13-16
    Average 90 stars, based on 1 article reviews
    mouse monoclonal anti-brafv600e antibody, clone ve1 - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Immunohistochemical staining:

    Article Title: BRAFV600E immunopositive Melanomas Show Low Frequency of Heterogeneity and Association With Epithelioid Tumor Cells
    Article Snippet: In brief, antigen retrieval was performed with EDTA (pH 9) using the PT link (Dako) for 10 minutes at 97 8C, subsequently followed by 5 minutes blocking with EnVision FLEX Peroxidase-Blocking Reagent, 20-minutes primary antibody incubation with ‘‘Mouse Anti-Human BRAFV600E Monoclonal Antibody VE1 (E19292—Spring Bioscience [1:50 dilution]), 15 minutes incubation with EnVision FLEXþMouse (Linker) and 20 minutes incubation with Envision FLEX HRP-labeled polymer.

    Article Title: Clinicopathologic correlations of the BRAFV600E mutation, BRAF V600E immunohistochemistry, and BRAF RNA in situ hybridization in papillary thyroid carcinoma.
    Article Snippet: Background: The BRAFV600E mutation is the most common genetic alteration in papillary thyroid carcinoma (PTC).. The aim of this study is to analyze the clinicopathologic correlations of the BRAFV600E mutation, BRAF V600E immunohistochemistry (IHC) and BRAF RNA in situ hybridization (ISH) in PTC.. Methods: This study included 467 patients with PTC who underwent surgical resection.

    Article Title: Syringocystadenoma papilliferum associated with verrucous carcinoma of the skin in the same lesion: Report of four cases.
    Article Snippet: The association of syringocystadenoma papilliferum with verrucous carcinoma of the skin in the same lesion is a rare, but well-documented event.. Although human papilloma viruses have been proposed to have an etiologic role in the development of the verrucous proliferations associated with syringocystadenoma papilliferum, most of the immunohistochemical and molecular studies have failed to demonstrate the presence of their genomic material in these lesions.. We report a series of 4 cases of syringocystadenoma papilliferum associated with verrucous carcinoma in anogenital lesions.

    Article Title: The landscape of BRAF transcript and protein variants in human cancer
    Article Snippet: They were then incubated overnight at 4 °C with the following primary antibodies: anti-BRAF (F-7, #5284, Santa Cruz Biotechnology; mouse monoclonal antibody, dilution 1:1000 in 5% milk in TBST); anti-human BRAFV600E (VE1, #E19290, Spring Bioscience; mouse monoclonal antibody, dilution 1:400 in 1% milk in TBST); anti-pMEK (#9154, Cell Signaling; rabbit monoclonal antibody, dilution 1:1000 in 3% BSA in TBST); anti-α-TUBULIN (#T9026, Sigma-Aldrich; mouse monoclonal antibody, dilution 1:20000 in 5% milk in TBST); anti-EGFP (#A111-22, Molecular Probes, rabbit polyclonal antibody, dilution 1:2000 in 5% milk in TBST).

    Article Title: Investigation of FOXM1 as a Potential New Target for Melanoma
    Article Snippet: Sections of paraffin-embedded melanomas and nevus tissue samples were stained with a monoclonal mouse anti-FOXM1 antibody (clone 3A9; Abnova, Taipei, Taiwan), monoclonal mouse anti-BRAFV600E antibody (clone VE1; Spring Bioscience, Pleasanton, CA) and monoclonal rabbit anti-phospho-AKT (Ser473) antibody (Cell Signaling Technologies, Tokyo, Japan).

    Article Title: Sessile Serrated Polyps are Precursors of Colon Carcinomas Predominantly with Deficient DNA Mismatch Repair
    Article Snippet: Staining for BRAF was performed with a pan-BRAF antibody (pBR1 clone; Spring Bioscience, Inc., Pleasanton, CA) diluted 1:100 at 37°C for 16 min. We then used an anti- BRAFV600E mouse monoclonal antibody (VE1 clone; Spring Bioscience, Inc.) raised against an immunogenic synthetic peptide derived from the internal region of the BRAFV600E protein.

    Article Title: Immunohistochemistry as an accurate tool for evaluating BRAF-V600E mutation in 130 samples of papillary thyroid cancer.
    Article Snippet: Background.. BRAF mutation has been investigated by immunohistochemistry and has shown high sensitivity and specificity.. We aim to investigate the accuracy of immunohistochemistry versus molecular testing of BRAF in papillary thyroid cancer using a large number of polymerase chain reaction–positive BRAF papillary thyroid cancer tissues.

    Article Title: BRAF V600E immunopositive Melanomas Show Low Frequency of Heterogeneity and Association With Epithelioid Tumor Cells
    Article Snippet: In brief, antigen retrieval was performed with EDTA (pH 9) using the PT link (Dako) for 10 minutes at 97 °C, subsequently followed by 5 minutes blocking with EnVision FLEX Peroxidase-Blocking Reagent, 20-minutes primary antibody incubation with “Mouse Anti-Human BRAFV600E Monoclonal Antibody VE1 (E19292—Spring Bioscience [1:50 dilution]), 15 minutes incubation with EnVision FLEX + Mouse (Linker) and 20 minutes incubation with Envision FLEX HRP-labeled polymer.

    Expressing:

    Article Title: BRAFV600E immunopositive Melanomas Show Low Frequency of Heterogeneity and Association With Epithelioid Tumor Cells
    Article Snippet: In brief, antigen retrieval was performed with EDTA (pH 9) using the PT link (Dako) for 10 minutes at 97 8C, subsequently followed by 5 minutes blocking with EnVision FLEX Peroxidase-Blocking Reagent, 20-minutes primary antibody incubation with ‘‘Mouse Anti-Human BRAFV600E Monoclonal Antibody VE1 (E19292—Spring Bioscience [1:50 dilution]), 15 minutes incubation with EnVision FLEXþMouse (Linker) and 20 minutes incubation with Envision FLEX HRP-labeled polymer.

    Article Title: Clinicopathologic correlations of the BRAFV600E mutation, BRAF V600E immunohistochemistry, and BRAF RNA in situ hybridization in papillary thyroid carcinoma.
    Article Snippet: Background: The BRAFV600E mutation is the most common genetic alteration in papillary thyroid carcinoma (PTC).. The aim of this study is to analyze the clinicopathologic correlations of the BRAFV600E mutation, BRAF V600E immunohistochemistry (IHC) and BRAF RNA in situ hybridization (ISH) in PTC.. Methods: This study included 467 patients with PTC who underwent surgical resection.

    Article Title: Syringocystadenoma papilliferum associated with verrucous carcinoma of the skin in the same lesion: Report of four cases.
    Article Snippet: The association of syringocystadenoma papilliferum with verrucous carcinoma of the skin in the same lesion is a rare, but well-documented event.. Although human papilloma viruses have been proposed to have an etiologic role in the development of the verrucous proliferations associated with syringocystadenoma papilliferum, most of the immunohistochemical and molecular studies have failed to demonstrate the presence of their genomic material in these lesions.. We report a series of 4 cases of syringocystadenoma papilliferum associated with verrucous carcinoma in anogenital lesions.

    Article Title: The landscape of BRAF transcript and protein variants in human cancer
    Article Snippet: They were then incubated overnight at 4 °C with the following primary antibodies: anti-BRAF (F-7, #5284, Santa Cruz Biotechnology; mouse monoclonal antibody, dilution 1:1000 in 5% milk in TBST); anti-human BRAFV600E (VE1, #E19290, Spring Bioscience; mouse monoclonal antibody, dilution 1:400 in 1% milk in TBST); anti-pMEK (#9154, Cell Signaling; rabbit monoclonal antibody, dilution 1:1000 in 3% BSA in TBST); anti-α-TUBULIN (#T9026, Sigma-Aldrich; mouse monoclonal antibody, dilution 1:20000 in 5% milk in TBST); anti-EGFP (#A111-22, Molecular Probes, rabbit polyclonal antibody, dilution 1:2000 in 5% milk in TBST).

    Article Title: Investigation of FOXM1 as a Potential New Target for Melanoma
    Article Snippet: Sections of paraffin-embedded melanomas and nevus tissue samples were stained with a monoclonal mouse anti-FOXM1 antibody (clone 3A9; Abnova, Taipei, Taiwan), monoclonal mouse anti-BRAFV600E antibody (clone VE1; Spring Bioscience, Pleasanton, CA) and monoclonal rabbit anti-phospho-AKT (Ser473) antibody (Cell Signaling Technologies, Tokyo, Japan).

    Article Title: Sessile Serrated Polyps are Precursors of Colon Carcinomas Predominantly with Deficient DNA Mismatch Repair
    Article Snippet: Staining for BRAF was performed with a pan-BRAF antibody (pBR1 clone; Spring Bioscience, Inc., Pleasanton, CA) diluted 1:100 at 37°C for 16 min. We then used an anti- BRAFV600E mouse monoclonal antibody (VE1 clone; Spring Bioscience, Inc.) raised against an immunogenic synthetic peptide derived from the internal region of the BRAFV600E protein.

    Article Title: Immunohistochemistry as an accurate tool for evaluating BRAF-V600E mutation in 130 samples of papillary thyroid cancer.
    Article Snippet: Background.. BRAF mutation has been investigated by immunohistochemistry and has shown high sensitivity and specificity.. We aim to investigate the accuracy of immunohistochemistry versus molecular testing of BRAF in papillary thyroid cancer using a large number of polymerase chain reaction–positive BRAF papillary thyroid cancer tissues.

    Article Title: BRAF V600E immunopositive Melanomas Show Low Frequency of Heterogeneity and Association With Epithelioid Tumor Cells
    Article Snippet: In brief, antigen retrieval was performed with EDTA (pH 9) using the PT link (Dako) for 10 minutes at 97 °C, subsequently followed by 5 minutes blocking with EnVision FLEX Peroxidase-Blocking Reagent, 20-minutes primary antibody incubation with “Mouse Anti-Human BRAFV600E Monoclonal Antibody VE1 (E19292—Spring Bioscience [1:50 dilution]), 15 minutes incubation with EnVision FLEX + Mouse (Linker) and 20 minutes incubation with Envision FLEX HRP-labeled polymer.

    Staining:

    Article Title: BRAFV600E immunopositive Melanomas Show Low Frequency of Heterogeneity and Association With Epithelioid Tumor Cells
    Article Snippet: In brief, antigen retrieval was performed with EDTA (pH 9) using the PT link (Dako) for 10 minutes at 97 8C, subsequently followed by 5 minutes blocking with EnVision FLEX Peroxidase-Blocking Reagent, 20-minutes primary antibody incubation with ‘‘Mouse Anti-Human BRAFV600E Monoclonal Antibody VE1 (E19292—Spring Bioscience [1:50 dilution]), 15 minutes incubation with EnVision FLEXþMouse (Linker) and 20 minutes incubation with Envision FLEX HRP-labeled polymer.

    Article Title: Clinicopathologic correlations of the BRAFV600E mutation, BRAF V600E immunohistochemistry, and BRAF RNA in situ hybridization in papillary thyroid carcinoma.
    Article Snippet: Background: The BRAFV600E mutation is the most common genetic alteration in papillary thyroid carcinoma (PTC).. The aim of this study is to analyze the clinicopathologic correlations of the BRAFV600E mutation, BRAF V600E immunohistochemistry (IHC) and BRAF RNA in situ hybridization (ISH) in PTC.. Methods: This study included 467 patients with PTC who underwent surgical resection.

    Article Title: Syringocystadenoma papilliferum associated with verrucous carcinoma of the skin in the same lesion: Report of four cases.
    Article Snippet: The association of syringocystadenoma papilliferum with verrucous carcinoma of the skin in the same lesion is a rare, but well-documented event.. Although human papilloma viruses have been proposed to have an etiologic role in the development of the verrucous proliferations associated with syringocystadenoma papilliferum, most of the immunohistochemical and molecular studies have failed to demonstrate the presence of their genomic material in these lesions.. We report a series of 4 cases of syringocystadenoma papilliferum associated with verrucous carcinoma in anogenital lesions.

    Article Title: The landscape of BRAF transcript and protein variants in human cancer
    Article Snippet: They were then incubated overnight at 4 °C with the following primary antibodies: anti-BRAF (F-7, #5284, Santa Cruz Biotechnology; mouse monoclonal antibody, dilution 1:1000 in 5% milk in TBST); anti-human BRAFV600E (VE1, #E19290, Spring Bioscience; mouse monoclonal antibody, dilution 1:400 in 1% milk in TBST); anti-pMEK (#9154, Cell Signaling; rabbit monoclonal antibody, dilution 1:1000 in 3% BSA in TBST); anti-α-TUBULIN (#T9026, Sigma-Aldrich; mouse monoclonal antibody, dilution 1:20000 in 5% milk in TBST); anti-EGFP (#A111-22, Molecular Probes, rabbit polyclonal antibody, dilution 1:2000 in 5% milk in TBST).

    Article Title: Investigation of FOXM1 as a Potential New Target for Melanoma
    Article Snippet: Sections of paraffin-embedded melanomas and nevus tissue samples were stained with a monoclonal mouse anti-FOXM1 antibody (clone 3A9; Abnova, Taipei, Taiwan), monoclonal mouse anti-BRAFV600E antibody (clone VE1; Spring Bioscience, Pleasanton, CA) and monoclonal rabbit anti-phospho-AKT (Ser473) antibody (Cell Signaling Technologies, Tokyo, Japan).

    Article Title: Sessile Serrated Polyps are Precursors of Colon Carcinomas Predominantly with Deficient DNA Mismatch Repair
    Article Snippet: Staining for BRAF was performed with a pan-BRAF antibody (pBR1 clone; Spring Bioscience, Inc., Pleasanton, CA) diluted 1:100 at 37°C for 16 min. We then used an anti- BRAFV600E mouse monoclonal antibody (VE1 clone; Spring Bioscience, Inc.) raised against an immunogenic synthetic peptide derived from the internal region of the BRAFV600E protein.

    Article Title: Immunohistochemistry as an accurate tool for evaluating BRAF-V600E mutation in 130 samples of papillary thyroid cancer.
    Article Snippet: Background.. BRAF mutation has been investigated by immunohistochemistry and has shown high sensitivity and specificity.. We aim to investigate the accuracy of immunohistochemistry versus molecular testing of BRAF in papillary thyroid cancer using a large number of polymerase chain reaction–positive BRAF papillary thyroid cancer tissues.

    Article Title: BRAF V600E immunopositive Melanomas Show Low Frequency of Heterogeneity and Association With Epithelioid Tumor Cells
    Article Snippet: In brief, antigen retrieval was performed with EDTA (pH 9) using the PT link (Dako) for 10 minutes at 97 °C, subsequently followed by 5 minutes blocking with EnVision FLEX Peroxidase-Blocking Reagent, 20-minutes primary antibody incubation with “Mouse Anti-Human BRAFV600E Monoclonal Antibody VE1 (E19292—Spring Bioscience [1:50 dilution]), 15 minutes incubation with EnVision FLEX + Mouse (Linker) and 20 minutes incubation with Envision FLEX HRP-labeled polymer.

    Derivative Assay:

    Article Title: BRAFV600E immunopositive Melanomas Show Low Frequency of Heterogeneity and Association With Epithelioid Tumor Cells
    Article Snippet: In brief, antigen retrieval was performed with EDTA (pH 9) using the PT link (Dako) for 10 minutes at 97 8C, subsequently followed by 5 minutes blocking with EnVision FLEX Peroxidase-Blocking Reagent, 20-minutes primary antibody incubation with ‘‘Mouse Anti-Human BRAFV600E Monoclonal Antibody VE1 (E19292—Spring Bioscience [1:50 dilution]), 15 minutes incubation with EnVision FLEXþMouse (Linker) and 20 minutes incubation with Envision FLEX HRP-labeled polymer.

    Article Title: Clinicopathologic correlations of the BRAFV600E mutation, BRAF V600E immunohistochemistry, and BRAF RNA in situ hybridization in papillary thyroid carcinoma.
    Article Snippet: Background: The BRAFV600E mutation is the most common genetic alteration in papillary thyroid carcinoma (PTC).. The aim of this study is to analyze the clinicopathologic correlations of the BRAFV600E mutation, BRAF V600E immunohistochemistry (IHC) and BRAF RNA in situ hybridization (ISH) in PTC.. Methods: This study included 467 patients with PTC who underwent surgical resection.

    Article Title: Syringocystadenoma papilliferum associated with verrucous carcinoma of the skin in the same lesion: Report of four cases.
    Article Snippet: The association of syringocystadenoma papilliferum with verrucous carcinoma of the skin in the same lesion is a rare, but well-documented event.. Although human papilloma viruses have been proposed to have an etiologic role in the development of the verrucous proliferations associated with syringocystadenoma papilliferum, most of the immunohistochemical and molecular studies have failed to demonstrate the presence of their genomic material in these lesions.. We report a series of 4 cases of syringocystadenoma papilliferum associated with verrucous carcinoma in anogenital lesions.

    Article Title: The landscape of BRAF transcript and protein variants in human cancer
    Article Snippet: They were then incubated overnight at 4 °C with the following primary antibodies: anti-BRAF (F-7, #5284, Santa Cruz Biotechnology; mouse monoclonal antibody, dilution 1:1000 in 5% milk in TBST); anti-human BRAFV600E (VE1, #E19290, Spring Bioscience; mouse monoclonal antibody, dilution 1:400 in 1% milk in TBST); anti-pMEK (#9154, Cell Signaling; rabbit monoclonal antibody, dilution 1:1000 in 3% BSA in TBST); anti-α-TUBULIN (#T9026, Sigma-Aldrich; mouse monoclonal antibody, dilution 1:20000 in 5% milk in TBST); anti-EGFP (#A111-22, Molecular Probes, rabbit polyclonal antibody, dilution 1:2000 in 5% milk in TBST).

    Article Title: Investigation of FOXM1 as a Potential New Target for Melanoma
    Article Snippet: Sections of paraffin-embedded melanomas and nevus tissue samples were stained with a monoclonal mouse anti-FOXM1 antibody (clone 3A9; Abnova, Taipei, Taiwan), monoclonal mouse anti-BRAFV600E antibody (clone VE1; Spring Bioscience, Pleasanton, CA) and monoclonal rabbit anti-phospho-AKT (Ser473) antibody (Cell Signaling Technologies, Tokyo, Japan).

    Article Title: Sessile Serrated Polyps are Precursors of Colon Carcinomas Predominantly with Deficient DNA Mismatch Repair
    Article Snippet: Staining for BRAF was performed with a pan-BRAF antibody (pBR1 clone; Spring Bioscience, Inc., Pleasanton, CA) diluted 1:100 at 37°C for 16 min. We then used an anti- BRAFV600E mouse monoclonal antibody (VE1 clone; Spring Bioscience, Inc.) raised against an immunogenic synthetic peptide derived from the internal region of the BRAFV600E protein.

    Article Title: Immunohistochemistry as an accurate tool for evaluating BRAF-V600E mutation in 130 samples of papillary thyroid cancer.
    Article Snippet: Background.. BRAF mutation has been investigated by immunohistochemistry and has shown high sensitivity and specificity.. We aim to investigate the accuracy of immunohistochemistry versus molecular testing of BRAF in papillary thyroid cancer using a large number of polymerase chain reaction–positive BRAF papillary thyroid cancer tissues.

    Article Title: BRAF V600E immunopositive Melanomas Show Low Frequency of Heterogeneity and Association With Epithelioid Tumor Cells
    Article Snippet: In brief, antigen retrieval was performed with EDTA (pH 9) using the PT link (Dako) for 10 minutes at 97 °C, subsequently followed by 5 minutes blocking with EnVision FLEX Peroxidase-Blocking Reagent, 20-minutes primary antibody incubation with “Mouse Anti-Human BRAFV600E Monoclonal Antibody VE1 (E19292—Spring Bioscience [1:50 dilution]), 15 minutes incubation with EnVision FLEX + Mouse (Linker) and 20 minutes incubation with Envision FLEX HRP-labeled polymer.

    Incubation:

    Article Title: BRAFV600E immunopositive Melanomas Show Low Frequency of Heterogeneity and Association With Epithelioid Tumor Cells
    Article Snippet: In brief, antigen retrieval was performed with EDTA (pH 9) using the PT link (Dako) for 10 minutes at 97 8C, subsequently followed by 5 minutes blocking with EnVision FLEX Peroxidase-Blocking Reagent, 20-minutes primary antibody incubation with ‘‘Mouse Anti-Human BRAFV600E Monoclonal Antibody VE1 (E19292—Spring Bioscience [1:50 dilution]), 15 minutes incubation with EnVision FLEXþMouse (Linker) and 20 minutes incubation with Envision FLEX HRP-labeled polymer.

    Article Title: Clinicopathologic correlations of the BRAFV600E mutation, BRAF V600E immunohistochemistry, and BRAF RNA in situ hybridization in papillary thyroid carcinoma.
    Article Snippet: Background: The BRAFV600E mutation is the most common genetic alteration in papillary thyroid carcinoma (PTC).. The aim of this study is to analyze the clinicopathologic correlations of the BRAFV600E mutation, BRAF V600E immunohistochemistry (IHC) and BRAF RNA in situ hybridization (ISH) in PTC.. Methods: This study included 467 patients with PTC who underwent surgical resection.

    Article Title: Syringocystadenoma papilliferum associated with verrucous carcinoma of the skin in the same lesion: Report of four cases.
    Article Snippet: The association of syringocystadenoma papilliferum with verrucous carcinoma of the skin in the same lesion is a rare, but well-documented event.. Although human papilloma viruses have been proposed to have an etiologic role in the development of the verrucous proliferations associated with syringocystadenoma papilliferum, most of the immunohistochemical and molecular studies have failed to demonstrate the presence of their genomic material in these lesions.. We report a series of 4 cases of syringocystadenoma papilliferum associated with verrucous carcinoma in anogenital lesions.

    Article Title: The landscape of BRAF transcript and protein variants in human cancer
    Article Snippet: They were then incubated overnight at 4 °C with the following primary antibodies: anti-BRAF (F-7, #5284, Santa Cruz Biotechnology; mouse monoclonal antibody, dilution 1:1000 in 5% milk in TBST); anti-human BRAFV600E (VE1, #E19290, Spring Bioscience; mouse monoclonal antibody, dilution 1:400 in 1% milk in TBST); anti-pMEK (#9154, Cell Signaling; rabbit monoclonal antibody, dilution 1:1000 in 3% BSA in TBST); anti-α-TUBULIN (#T9026, Sigma-Aldrich; mouse monoclonal antibody, dilution 1:20000 in 5% milk in TBST); anti-EGFP (#A111-22, Molecular Probes, rabbit polyclonal antibody, dilution 1:2000 in 5% milk in TBST).

    Article Title: Investigation of FOXM1 as a Potential New Target for Melanoma
    Article Snippet: Sections of paraffin-embedded melanomas and nevus tissue samples were stained with a monoclonal mouse anti-FOXM1 antibody (clone 3A9; Abnova, Taipei, Taiwan), monoclonal mouse anti-BRAFV600E antibody (clone VE1; Spring Bioscience, Pleasanton, CA) and monoclonal rabbit anti-phospho-AKT (Ser473) antibody (Cell Signaling Technologies, Tokyo, Japan).

    Article Title: Sessile Serrated Polyps are Precursors of Colon Carcinomas Predominantly with Deficient DNA Mismatch Repair
    Article Snippet: Staining for BRAF was performed with a pan-BRAF antibody (pBR1 clone; Spring Bioscience, Inc., Pleasanton, CA) diluted 1:100 at 37°C for 16 min. We then used an anti- BRAFV600E mouse monoclonal antibody (VE1 clone; Spring Bioscience, Inc.) raised against an immunogenic synthetic peptide derived from the internal region of the BRAFV600E protein.

    Article Title: Immunohistochemistry as an accurate tool for evaluating BRAF-V600E mutation in 130 samples of papillary thyroid cancer.
    Article Snippet: Background.. BRAF mutation has been investigated by immunohistochemistry and has shown high sensitivity and specificity.. We aim to investigate the accuracy of immunohistochemistry versus molecular testing of BRAF in papillary thyroid cancer using a large number of polymerase chain reaction–positive BRAF papillary thyroid cancer tissues.

    Article Title: BRAF V600E immunopositive Melanomas Show Low Frequency of Heterogeneity and Association With Epithelioid Tumor Cells
    Article Snippet: In brief, antigen retrieval was performed with EDTA (pH 9) using the PT link (Dako) for 10 minutes at 97 °C, subsequently followed by 5 minutes blocking with EnVision FLEX Peroxidase-Blocking Reagent, 20-minutes primary antibody incubation with “Mouse Anti-Human BRAFV600E Monoclonal Antibody VE1 (E19292—Spring Bioscience [1:50 dilution]), 15 minutes incubation with EnVision FLEX + Mouse (Linker) and 20 minutes incubation with Envision FLEX HRP-labeled polymer.

    Real-time Polymerase Chain Reaction:

    Article Title: BRAFV600E immunopositive Melanomas Show Low Frequency of Heterogeneity and Association With Epithelioid Tumor Cells
    Article Snippet: In brief, antigen retrieval was performed with EDTA (pH 9) using the PT link (Dako) for 10 minutes at 97 8C, subsequently followed by 5 minutes blocking with EnVision FLEX Peroxidase-Blocking Reagent, 20-minutes primary antibody incubation with ‘‘Mouse Anti-Human BRAFV600E Monoclonal Antibody VE1 (E19292—Spring Bioscience [1:50 dilution]), 15 minutes incubation with EnVision FLEXþMouse (Linker) and 20 minutes incubation with Envision FLEX HRP-labeled polymer.

    Article Title: Clinicopathologic correlations of the BRAFV600E mutation, BRAF V600E immunohistochemistry, and BRAF RNA in situ hybridization in papillary thyroid carcinoma.
    Article Snippet: Background: The BRAFV600E mutation is the most common genetic alteration in papillary thyroid carcinoma (PTC).. The aim of this study is to analyze the clinicopathologic correlations of the BRAFV600E mutation, BRAF V600E immunohistochemistry (IHC) and BRAF RNA in situ hybridization (ISH) in PTC.. Methods: This study included 467 patients with PTC who underwent surgical resection.

    Article Title: Syringocystadenoma papilliferum associated with verrucous carcinoma of the skin in the same lesion: Report of four cases.
    Article Snippet: The association of syringocystadenoma papilliferum with verrucous carcinoma of the skin in the same lesion is a rare, but well-documented event.. Although human papilloma viruses have been proposed to have an etiologic role in the development of the verrucous proliferations associated with syringocystadenoma papilliferum, most of the immunohistochemical and molecular studies have failed to demonstrate the presence of their genomic material in these lesions.. We report a series of 4 cases of syringocystadenoma papilliferum associated with verrucous carcinoma in anogenital lesions.

    Article Title: The landscape of BRAF transcript and protein variants in human cancer
    Article Snippet: They were then incubated overnight at 4 °C with the following primary antibodies: anti-BRAF (F-7, #5284, Santa Cruz Biotechnology; mouse monoclonal antibody, dilution 1:1000 in 5% milk in TBST); anti-human BRAFV600E (VE1, #E19290, Spring Bioscience; mouse monoclonal antibody, dilution 1:400 in 1% milk in TBST); anti-pMEK (#9154, Cell Signaling; rabbit monoclonal antibody, dilution 1:1000 in 3% BSA in TBST); anti-α-TUBULIN (#T9026, Sigma-Aldrich; mouse monoclonal antibody, dilution 1:20000 in 5% milk in TBST); anti-EGFP (#A111-22, Molecular Probes, rabbit polyclonal antibody, dilution 1:2000 in 5% milk in TBST).

    Article Title: Investigation of FOXM1 as a Potential New Target for Melanoma
    Article Snippet: Sections of paraffin-embedded melanomas and nevus tissue samples were stained with a monoclonal mouse anti-FOXM1 antibody (clone 3A9; Abnova, Taipei, Taiwan), monoclonal mouse anti-BRAFV600E antibody (clone VE1; Spring Bioscience, Pleasanton, CA) and monoclonal rabbit anti-phospho-AKT (Ser473) antibody (Cell Signaling Technologies, Tokyo, Japan).

    Article Title: Sessile Serrated Polyps are Precursors of Colon Carcinomas Predominantly with Deficient DNA Mismatch Repair
    Article Snippet: Staining for BRAF was performed with a pan-BRAF antibody (pBR1 clone; Spring Bioscience, Inc., Pleasanton, CA) diluted 1:100 at 37°C for 16 min. We then used an anti- BRAFV600E mouse monoclonal antibody (VE1 clone; Spring Bioscience, Inc.) raised against an immunogenic synthetic peptide derived from the internal region of the BRAFV600E protein.

    Article Title: Immunohistochemistry as an accurate tool for evaluating BRAF-V600E mutation in 130 samples of papillary thyroid cancer.
    Article Snippet: Background.. BRAF mutation has been investigated by immunohistochemistry and has shown high sensitivity and specificity.. We aim to investigate the accuracy of immunohistochemistry versus molecular testing of BRAF in papillary thyroid cancer using a large number of polymerase chain reaction–positive BRAF papillary thyroid cancer tissues.

    Article Title: BRAF V600E immunopositive Melanomas Show Low Frequency of Heterogeneity and Association With Epithelioid Tumor Cells
    Article Snippet: In brief, antigen retrieval was performed with EDTA (pH 9) using the PT link (Dako) for 10 minutes at 97 °C, subsequently followed by 5 minutes blocking with EnVision FLEX Peroxidase-Blocking Reagent, 20-minutes primary antibody incubation with “Mouse Anti-Human BRAFV600E Monoclonal Antibody VE1 (E19292—Spring Bioscience [1:50 dilution]), 15 minutes incubation with EnVision FLEX + Mouse (Linker) and 20 minutes incubation with Envision FLEX HRP-labeled polymer.

    Amplification:

    Article Title: BRAFV600E immunopositive Melanomas Show Low Frequency of Heterogeneity and Association With Epithelioid Tumor Cells
    Article Snippet: In brief, antigen retrieval was performed with EDTA (pH 9) using the PT link (Dako) for 10 minutes at 97 8C, subsequently followed by 5 minutes blocking with EnVision FLEX Peroxidase-Blocking Reagent, 20-minutes primary antibody incubation with ‘‘Mouse Anti-Human BRAFV600E Monoclonal Antibody VE1 (E19292—Spring Bioscience [1:50 dilution]), 15 minutes incubation with EnVision FLEXþMouse (Linker) and 20 minutes incubation with Envision FLEX HRP-labeled polymer.

    Article Title: Clinicopathologic correlations of the BRAFV600E mutation, BRAF V600E immunohistochemistry, and BRAF RNA in situ hybridization in papillary thyroid carcinoma.
    Article Snippet: Background: The BRAFV600E mutation is the most common genetic alteration in papillary thyroid carcinoma (PTC).. The aim of this study is to analyze the clinicopathologic correlations of the BRAFV600E mutation, BRAF V600E immunohistochemistry (IHC) and BRAF RNA in situ hybridization (ISH) in PTC.. Methods: This study included 467 patients with PTC who underwent surgical resection.

    Article Title: Syringocystadenoma papilliferum associated with verrucous carcinoma of the skin in the same lesion: Report of four cases.
    Article Snippet: The association of syringocystadenoma papilliferum with verrucous carcinoma of the skin in the same lesion is a rare, but well-documented event.. Although human papilloma viruses have been proposed to have an etiologic role in the development of the verrucous proliferations associated with syringocystadenoma papilliferum, most of the immunohistochemical and molecular studies have failed to demonstrate the presence of their genomic material in these lesions.. We report a series of 4 cases of syringocystadenoma papilliferum associated with verrucous carcinoma in anogenital lesions.

    Article Title: The landscape of BRAF transcript and protein variants in human cancer
    Article Snippet: They were then incubated overnight at 4 °C with the following primary antibodies: anti-BRAF (F-7, #5284, Santa Cruz Biotechnology; mouse monoclonal antibody, dilution 1:1000 in 5% milk in TBST); anti-human BRAFV600E (VE1, #E19290, Spring Bioscience; mouse monoclonal antibody, dilution 1:400 in 1% milk in TBST); anti-pMEK (#9154, Cell Signaling; rabbit monoclonal antibody, dilution 1:1000 in 3% BSA in TBST); anti-α-TUBULIN (#T9026, Sigma-Aldrich; mouse monoclonal antibody, dilution 1:20000 in 5% milk in TBST); anti-EGFP (#A111-22, Molecular Probes, rabbit polyclonal antibody, dilution 1:2000 in 5% milk in TBST).

    Article Title: Investigation of FOXM1 as a Potential New Target for Melanoma
    Article Snippet: Sections of paraffin-embedded melanomas and nevus tissue samples were stained with a monoclonal mouse anti-FOXM1 antibody (clone 3A9; Abnova, Taipei, Taiwan), monoclonal mouse anti-BRAFV600E antibody (clone VE1; Spring Bioscience, Pleasanton, CA) and monoclonal rabbit anti-phospho-AKT (Ser473) antibody (Cell Signaling Technologies, Tokyo, Japan).

    Article Title: Sessile Serrated Polyps are Precursors of Colon Carcinomas Predominantly with Deficient DNA Mismatch Repair
    Article Snippet: Staining for BRAF was performed with a pan-BRAF antibody (pBR1 clone; Spring Bioscience, Inc., Pleasanton, CA) diluted 1:100 at 37°C for 16 min. We then used an anti- BRAFV600E mouse monoclonal antibody (VE1 clone; Spring Bioscience, Inc.) raised against an immunogenic synthetic peptide derived from the internal region of the BRAFV600E protein.

    Article Title: Immunohistochemistry as an accurate tool for evaluating BRAF-V600E mutation in 130 samples of papillary thyroid cancer.
    Article Snippet: Background.. BRAF mutation has been investigated by immunohistochemistry and has shown high sensitivity and specificity.. We aim to investigate the accuracy of immunohistochemistry versus molecular testing of BRAF in papillary thyroid cancer using a large number of polymerase chain reaction–positive BRAF papillary thyroid cancer tissues.

    Article Title: BRAF V600E immunopositive Melanomas Show Low Frequency of Heterogeneity and Association With Epithelioid Tumor Cells
    Article Snippet: In brief, antigen retrieval was performed with EDTA (pH 9) using the PT link (Dako) for 10 minutes at 97 °C, subsequently followed by 5 minutes blocking with EnVision FLEX Peroxidase-Blocking Reagent, 20-minutes primary antibody incubation with “Mouse Anti-Human BRAFV600E Monoclonal Antibody VE1 (E19292—Spring Bioscience [1:50 dilution]), 15 minutes incubation with EnVision FLEX + Mouse (Linker) and 20 minutes incubation with Envision FLEX HRP-labeled polymer.

    Variant Assay:

    Article Title: BRAFV600E immunopositive Melanomas Show Low Frequency of Heterogeneity and Association With Epithelioid Tumor Cells
    Article Snippet: In brief, antigen retrieval was performed with EDTA (pH 9) using the PT link (Dako) for 10 minutes at 97 8C, subsequently followed by 5 minutes blocking with EnVision FLEX Peroxidase-Blocking Reagent, 20-minutes primary antibody incubation with ‘‘Mouse Anti-Human BRAFV600E Monoclonal Antibody VE1 (E19292—Spring Bioscience [1:50 dilution]), 15 minutes incubation with EnVision FLEXþMouse (Linker) and 20 minutes incubation with Envision FLEX HRP-labeled polymer.

    Article Title: Clinicopathologic correlations of the BRAFV600E mutation, BRAF V600E immunohistochemistry, and BRAF RNA in situ hybridization in papillary thyroid carcinoma.
    Article Snippet: Background: The BRAFV600E mutation is the most common genetic alteration in papillary thyroid carcinoma (PTC).. The aim of this study is to analyze the clinicopathologic correlations of the BRAFV600E mutation, BRAF V600E immunohistochemistry (IHC) and BRAF RNA in situ hybridization (ISH) in PTC.. Methods: This study included 467 patients with PTC who underwent surgical resection.

    Article Title: Syringocystadenoma papilliferum associated with verrucous carcinoma of the skin in the same lesion: Report of four cases.
    Article Snippet: The association of syringocystadenoma papilliferum with verrucous carcinoma of the skin in the same lesion is a rare, but well-documented event.. Although human papilloma viruses have been proposed to have an etiologic role in the development of the verrucous proliferations associated with syringocystadenoma papilliferum, most of the immunohistochemical and molecular studies have failed to demonstrate the presence of their genomic material in these lesions.. We report a series of 4 cases of syringocystadenoma papilliferum associated with verrucous carcinoma in anogenital lesions.

    Article Title: The landscape of BRAF transcript and protein variants in human cancer
    Article Snippet: They were then incubated overnight at 4 °C with the following primary antibodies: anti-BRAF (F-7, #5284, Santa Cruz Biotechnology; mouse monoclonal antibody, dilution 1:1000 in 5% milk in TBST); anti-human BRAFV600E (VE1, #E19290, Spring Bioscience; mouse monoclonal antibody, dilution 1:400 in 1% milk in TBST); anti-pMEK (#9154, Cell Signaling; rabbit monoclonal antibody, dilution 1:1000 in 3% BSA in TBST); anti-α-TUBULIN (#T9026, Sigma-Aldrich; mouse monoclonal antibody, dilution 1:20000 in 5% milk in TBST); anti-EGFP (#A111-22, Molecular Probes, rabbit polyclonal antibody, dilution 1:2000 in 5% milk in TBST).

    Article Title: Investigation of FOXM1 as a Potential New Target for Melanoma
    Article Snippet: Sections of paraffin-embedded melanomas and nevus tissue samples were stained with a monoclonal mouse anti-FOXM1 antibody (clone 3A9; Abnova, Taipei, Taiwan), monoclonal mouse anti-BRAFV600E antibody (clone VE1; Spring Bioscience, Pleasanton, CA) and monoclonal rabbit anti-phospho-AKT (Ser473) antibody (Cell Signaling Technologies, Tokyo, Japan).

    Article Title: Sessile Serrated Polyps are Precursors of Colon Carcinomas Predominantly with Deficient DNA Mismatch Repair
    Article Snippet: Staining for BRAF was performed with a pan-BRAF antibody (pBR1 clone; Spring Bioscience, Inc., Pleasanton, CA) diluted 1:100 at 37°C for 16 min. We then used an anti- BRAFV600E mouse monoclonal antibody (VE1 clone; Spring Bioscience, Inc.) raised against an immunogenic synthetic peptide derived from the internal region of the BRAFV600E protein.

    Article Title: Immunohistochemistry as an accurate tool for evaluating BRAF-V600E mutation in 130 samples of papillary thyroid cancer.
    Article Snippet: Background.. BRAF mutation has been investigated by immunohistochemistry and has shown high sensitivity and specificity.. We aim to investigate the accuracy of immunohistochemistry versus molecular testing of BRAF in papillary thyroid cancer using a large number of polymerase chain reaction–positive BRAF papillary thyroid cancer tissues.

    Article Title: BRAF V600E immunopositive Melanomas Show Low Frequency of Heterogeneity and Association With Epithelioid Tumor Cells
    Article Snippet: In brief, antigen retrieval was performed with EDTA (pH 9) using the PT link (Dako) for 10 minutes at 97 °C, subsequently followed by 5 minutes blocking with EnVision FLEX Peroxidase-Blocking Reagent, 20-minutes primary antibody incubation with “Mouse Anti-Human BRAFV600E Monoclonal Antibody VE1 (E19292—Spring Bioscience [1:50 dilution]), 15 minutes incubation with EnVision FLEX + Mouse (Linker) and 20 minutes incubation with Envision FLEX HRP-labeled polymer.

    Plasmid Preparation:

    Article Title: BRAFV600E immunopositive Melanomas Show Low Frequency of Heterogeneity and Association With Epithelioid Tumor Cells
    Article Snippet: In brief, antigen retrieval was performed with EDTA (pH 9) using the PT link (Dako) for 10 minutes at 97 8C, subsequently followed by 5 minutes blocking with EnVision FLEX Peroxidase-Blocking Reagent, 20-minutes primary antibody incubation with ‘‘Mouse Anti-Human BRAFV600E Monoclonal Antibody VE1 (E19292—Spring Bioscience [1:50 dilution]), 15 minutes incubation with EnVision FLEXþMouse (Linker) and 20 minutes incubation with Envision FLEX HRP-labeled polymer.

    Article Title: Clinicopathologic correlations of the BRAFV600E mutation, BRAF V600E immunohistochemistry, and BRAF RNA in situ hybridization in papillary thyroid carcinoma.
    Article Snippet: Background: The BRAFV600E mutation is the most common genetic alteration in papillary thyroid carcinoma (PTC).. The aim of this study is to analyze the clinicopathologic correlations of the BRAFV600E mutation, BRAF V600E immunohistochemistry (IHC) and BRAF RNA in situ hybridization (ISH) in PTC.. Methods: This study included 467 patients with PTC who underwent surgical resection.

    Article Title: Syringocystadenoma papilliferum associated with verrucous carcinoma of the skin in the same lesion: Report of four cases.
    Article Snippet: The association of syringocystadenoma papilliferum with verrucous carcinoma of the skin in the same lesion is a rare, but well-documented event.. Although human papilloma viruses have been proposed to have an etiologic role in the development of the verrucous proliferations associated with syringocystadenoma papilliferum, most of the immunohistochemical and molecular studies have failed to demonstrate the presence of their genomic material in these lesions.. We report a series of 4 cases of syringocystadenoma papilliferum associated with verrucous carcinoma in anogenital lesions.

    Article Title: The landscape of BRAF transcript and protein variants in human cancer
    Article Snippet: They were then incubated overnight at 4 °C with the following primary antibodies: anti-BRAF (F-7, #5284, Santa Cruz Biotechnology; mouse monoclonal antibody, dilution 1:1000 in 5% milk in TBST); anti-human BRAFV600E (VE1, #E19290, Spring Bioscience; mouse monoclonal antibody, dilution 1:400 in 1% milk in TBST); anti-pMEK (#9154, Cell Signaling; rabbit monoclonal antibody, dilution 1:1000 in 3% BSA in TBST); anti-α-TUBULIN (#T9026, Sigma-Aldrich; mouse monoclonal antibody, dilution 1:20000 in 5% milk in TBST); anti-EGFP (#A111-22, Molecular Probes, rabbit polyclonal antibody, dilution 1:2000 in 5% milk in TBST).

    Article Title: Investigation of FOXM1 as a Potential New Target for Melanoma
    Article Snippet: Sections of paraffin-embedded melanomas and nevus tissue samples were stained with a monoclonal mouse anti-FOXM1 antibody (clone 3A9; Abnova, Taipei, Taiwan), monoclonal mouse anti-BRAFV600E antibody (clone VE1; Spring Bioscience, Pleasanton, CA) and monoclonal rabbit anti-phospho-AKT (Ser473) antibody (Cell Signaling Technologies, Tokyo, Japan).

    Article Title: Sessile Serrated Polyps are Precursors of Colon Carcinomas Predominantly with Deficient DNA Mismatch Repair
    Article Snippet: Staining for BRAF was performed with a pan-BRAF antibody (pBR1 clone; Spring Bioscience, Inc., Pleasanton, CA) diluted 1:100 at 37°C for 16 min. We then used an anti- BRAFV600E mouse monoclonal antibody (VE1 clone; Spring Bioscience, Inc.) raised against an immunogenic synthetic peptide derived from the internal region of the BRAFV600E protein.

    Article Title: Immunohistochemistry as an accurate tool for evaluating BRAF-V600E mutation in 130 samples of papillary thyroid cancer.
    Article Snippet: Background.. BRAF mutation has been investigated by immunohistochemistry and has shown high sensitivity and specificity.. We aim to investigate the accuracy of immunohistochemistry versus molecular testing of BRAF in papillary thyroid cancer using a large number of polymerase chain reaction–positive BRAF papillary thyroid cancer tissues.

    Article Title: BRAF V600E immunopositive Melanomas Show Low Frequency of Heterogeneity and Association With Epithelioid Tumor Cells
    Article Snippet: In brief, antigen retrieval was performed with EDTA (pH 9) using the PT link (Dako) for 10 minutes at 97 °C, subsequently followed by 5 minutes blocking with EnVision FLEX Peroxidase-Blocking Reagent, 20-minutes primary antibody incubation with “Mouse Anti-Human BRAFV600E Monoclonal Antibody VE1 (E19292—Spring Bioscience [1:50 dilution]), 15 minutes incubation with EnVision FLEX + Mouse (Linker) and 20 minutes incubation with Envision FLEX HRP-labeled polymer.

    Positive Control:

    Article Title: BRAFV600E immunopositive Melanomas Show Low Frequency of Heterogeneity and Association With Epithelioid Tumor Cells
    Article Snippet: In brief, antigen retrieval was performed with EDTA (pH 9) using the PT link (Dako) for 10 minutes at 97 8C, subsequently followed by 5 minutes blocking with EnVision FLEX Peroxidase-Blocking Reagent, 20-minutes primary antibody incubation with ‘‘Mouse Anti-Human BRAFV600E Monoclonal Antibody VE1 (E19292—Spring Bioscience [1:50 dilution]), 15 minutes incubation with EnVision FLEXþMouse (Linker) and 20 minutes incubation with Envision FLEX HRP-labeled polymer.

    Article Title: Clinicopathologic correlations of the BRAFV600E mutation, BRAF V600E immunohistochemistry, and BRAF RNA in situ hybridization in papillary thyroid carcinoma.
    Article Snippet: Background: The BRAFV600E mutation is the most common genetic alteration in papillary thyroid carcinoma (PTC).. The aim of this study is to analyze the clinicopathologic correlations of the BRAFV600E mutation, BRAF V600E immunohistochemistry (IHC) and BRAF RNA in situ hybridization (ISH) in PTC.. Methods: This study included 467 patients with PTC who underwent surgical resection.

    Article Title: Syringocystadenoma papilliferum associated with verrucous carcinoma of the skin in the same lesion: Report of four cases.
    Article Snippet: The association of syringocystadenoma papilliferum with verrucous carcinoma of the skin in the same lesion is a rare, but well-documented event.. Although human papilloma viruses have been proposed to have an etiologic role in the development of the verrucous proliferations associated with syringocystadenoma papilliferum, most of the immunohistochemical and molecular studies have failed to demonstrate the presence of their genomic material in these lesions.. We report a series of 4 cases of syringocystadenoma papilliferum associated with verrucous carcinoma in anogenital lesions.

    Article Title: The landscape of BRAF transcript and protein variants in human cancer
    Article Snippet: They were then incubated overnight at 4 °C with the following primary antibodies: anti-BRAF (F-7, #5284, Santa Cruz Biotechnology; mouse monoclonal antibody, dilution 1:1000 in 5% milk in TBST); anti-human BRAFV600E (VE1, #E19290, Spring Bioscience; mouse monoclonal antibody, dilution 1:400 in 1% milk in TBST); anti-pMEK (#9154, Cell Signaling; rabbit monoclonal antibody, dilution 1:1000 in 3% BSA in TBST); anti-α-TUBULIN (#T9026, Sigma-Aldrich; mouse monoclonal antibody, dilution 1:20000 in 5% milk in TBST); anti-EGFP (#A111-22, Molecular Probes, rabbit polyclonal antibody, dilution 1:2000 in 5% milk in TBST).

    Article Title: Investigation of FOXM1 as a Potential New Target for Melanoma
    Article Snippet: Sections of paraffin-embedded melanomas and nevus tissue samples were stained with a monoclonal mouse anti-FOXM1 antibody (clone 3A9; Abnova, Taipei, Taiwan), monoclonal mouse anti-BRAFV600E antibody (clone VE1; Spring Bioscience, Pleasanton, CA) and monoclonal rabbit anti-phospho-AKT (Ser473) antibody (Cell Signaling Technologies, Tokyo, Japan).

    Article Title: Sessile Serrated Polyps are Precursors of Colon Carcinomas Predominantly with Deficient DNA Mismatch Repair
    Article Snippet: Staining for BRAF was performed with a pan-BRAF antibody (pBR1 clone; Spring Bioscience, Inc., Pleasanton, CA) diluted 1:100 at 37°C for 16 min. We then used an anti- BRAFV600E mouse monoclonal antibody (VE1 clone; Spring Bioscience, Inc.) raised against an immunogenic synthetic peptide derived from the internal region of the BRAFV600E protein.

    Article Title: Immunohistochemistry as an accurate tool for evaluating BRAF-V600E mutation in 130 samples of papillary thyroid cancer.
    Article Snippet: Background.. BRAF mutation has been investigated by immunohistochemistry and has shown high sensitivity and specificity.. We aim to investigate the accuracy of immunohistochemistry versus molecular testing of BRAF in papillary thyroid cancer using a large number of polymerase chain reaction–positive BRAF papillary thyroid cancer tissues.

    Article Title: BRAF V600E immunopositive Melanomas Show Low Frequency of Heterogeneity and Association With Epithelioid Tumor Cells
    Article Snippet: In brief, antigen retrieval was performed with EDTA (pH 9) using the PT link (Dako) for 10 minutes at 97 °C, subsequently followed by 5 minutes blocking with EnVision FLEX Peroxidase-Blocking Reagent, 20-minutes primary antibody incubation with “Mouse Anti-Human BRAFV600E Monoclonal Antibody VE1 (E19292—Spring Bioscience [1:50 dilution]), 15 minutes incubation with EnVision FLEX + Mouse (Linker) and 20 minutes incubation with Envision FLEX HRP-labeled polymer.

    Transfection:

    Article Title: BRAFV600E immunopositive Melanomas Show Low Frequency of Heterogeneity and Association With Epithelioid Tumor Cells
    Article Snippet: In brief, antigen retrieval was performed with EDTA (pH 9) using the PT link (Dako) for 10 minutes at 97 8C, subsequently followed by 5 minutes blocking with EnVision FLEX Peroxidase-Blocking Reagent, 20-minutes primary antibody incubation with ‘‘Mouse Anti-Human BRAFV600E Monoclonal Antibody VE1 (E19292—Spring Bioscience [1:50 dilution]), 15 minutes incubation with EnVision FLEXþMouse (Linker) and 20 minutes incubation with Envision FLEX HRP-labeled polymer.

    Article Title: Clinicopathologic correlations of the BRAFV600E mutation, BRAF V600E immunohistochemistry, and BRAF RNA in situ hybridization in papillary thyroid carcinoma.
    Article Snippet: Background: The BRAFV600E mutation is the most common genetic alteration in papillary thyroid carcinoma (PTC).. The aim of this study is to analyze the clinicopathologic correlations of the BRAFV600E mutation, BRAF V600E immunohistochemistry (IHC) and BRAF RNA in situ hybridization (ISH) in PTC.. Methods: This study included 467 patients with PTC who underwent surgical resection.

    Article Title: Syringocystadenoma papilliferum associated with verrucous carcinoma of the skin in the same lesion: Report of four cases.
    Article Snippet: The association of syringocystadenoma papilliferum with verrucous carcinoma of the skin in the same lesion is a rare, but well-documented event.. Although human papilloma viruses have been proposed to have an etiologic role in the development of the verrucous proliferations associated with syringocystadenoma papilliferum, most of the immunohistochemical and molecular studies have failed to demonstrate the presence of their genomic material in these lesions.. We report a series of 4 cases of syringocystadenoma papilliferum associated with verrucous carcinoma in anogenital lesions.

    Article Title: The landscape of BRAF transcript and protein variants in human cancer
    Article Snippet: They were then incubated overnight at 4 °C with the following primary antibodies: anti-BRAF (F-7, #5284, Santa Cruz Biotechnology; mouse monoclonal antibody, dilution 1:1000 in 5% milk in TBST); anti-human BRAFV600E (VE1, #E19290, Spring Bioscience; mouse monoclonal antibody, dilution 1:400 in 1% milk in TBST); anti-pMEK (#9154, Cell Signaling; rabbit monoclonal antibody, dilution 1:1000 in 3% BSA in TBST); anti-α-TUBULIN (#T9026, Sigma-Aldrich; mouse monoclonal antibody, dilution 1:20000 in 5% milk in TBST); anti-EGFP (#A111-22, Molecular Probes, rabbit polyclonal antibody, dilution 1:2000 in 5% milk in TBST).

    Article Title: Investigation of FOXM1 as a Potential New Target for Melanoma
    Article Snippet: Sections of paraffin-embedded melanomas and nevus tissue samples were stained with a monoclonal mouse anti-FOXM1 antibody (clone 3A9; Abnova, Taipei, Taiwan), monoclonal mouse anti-BRAFV600E antibody (clone VE1; Spring Bioscience, Pleasanton, CA) and monoclonal rabbit anti-phospho-AKT (Ser473) antibody (Cell Signaling Technologies, Tokyo, Japan).

    Article Title: Sessile Serrated Polyps are Precursors of Colon Carcinomas Predominantly with Deficient DNA Mismatch Repair
    Article Snippet: Staining for BRAF was performed with a pan-BRAF antibody (pBR1 clone; Spring Bioscience, Inc., Pleasanton, CA) diluted 1:100 at 37°C for 16 min. We then used an anti- BRAFV600E mouse monoclonal antibody (VE1 clone; Spring Bioscience, Inc.) raised against an immunogenic synthetic peptide derived from the internal region of the BRAFV600E protein.

    Article Title: Immunohistochemistry as an accurate tool for evaluating BRAF-V600E mutation in 130 samples of papillary thyroid cancer.
    Article Snippet: Background.. BRAF mutation has been investigated by immunohistochemistry and has shown high sensitivity and specificity.. We aim to investigate the accuracy of immunohistochemistry versus molecular testing of BRAF in papillary thyroid cancer using a large number of polymerase chain reaction–positive BRAF papillary thyroid cancer tissues.

    Article Title: BRAF V600E immunopositive Melanomas Show Low Frequency of Heterogeneity and Association With Epithelioid Tumor Cells
    Article Snippet: In brief, antigen retrieval was performed with EDTA (pH 9) using the PT link (Dako) for 10 minutes at 97 °C, subsequently followed by 5 minutes blocking with EnVision FLEX Peroxidase-Blocking Reagent, 20-minutes primary antibody incubation with “Mouse Anti-Human BRAFV600E Monoclonal Antibody VE1 (E19292—Spring Bioscience [1:50 dilution]), 15 minutes incubation with EnVision FLEX + Mouse (Linker) and 20 minutes incubation with Envision FLEX HRP-labeled polymer.

    Western Blot:

    Article Title: BRAFV600E immunopositive Melanomas Show Low Frequency of Heterogeneity and Association With Epithelioid Tumor Cells
    Article Snippet: In brief, antigen retrieval was performed with EDTA (pH 9) using the PT link (Dako) for 10 minutes at 97 8C, subsequently followed by 5 minutes blocking with EnVision FLEX Peroxidase-Blocking Reagent, 20-minutes primary antibody incubation with ‘‘Mouse Anti-Human BRAFV600E Monoclonal Antibody VE1 (E19292—Spring Bioscience [1:50 dilution]), 15 minutes incubation with EnVision FLEXþMouse (Linker) and 20 minutes incubation with Envision FLEX HRP-labeled polymer.

    Article Title: Clinicopathologic correlations of the BRAFV600E mutation, BRAF V600E immunohistochemistry, and BRAF RNA in situ hybridization in papillary thyroid carcinoma.
    Article Snippet: Background: The BRAFV600E mutation is the most common genetic alteration in papillary thyroid carcinoma (PTC).. The aim of this study is to analyze the clinicopathologic correlations of the BRAFV600E mutation, BRAF V600E immunohistochemistry (IHC) and BRAF RNA in situ hybridization (ISH) in PTC.. Methods: This study included 467 patients with PTC who underwent surgical resection.

    Article Title: Syringocystadenoma papilliferum associated with verrucous carcinoma of the skin in the same lesion: Report of four cases.
    Article Snippet: The association of syringocystadenoma papilliferum with verrucous carcinoma of the skin in the same lesion is a rare, but well-documented event.. Although human papilloma viruses have been proposed to have an etiologic role in the development of the verrucous proliferations associated with syringocystadenoma papilliferum, most of the immunohistochemical and molecular studies have failed to demonstrate the presence of their genomic material in these lesions.. We report a series of 4 cases of syringocystadenoma papilliferum associated with verrucous carcinoma in anogenital lesions.

    Article Title: The landscape of BRAF transcript and protein variants in human cancer
    Article Snippet: They were then incubated overnight at 4 °C with the following primary antibodies: anti-BRAF (F-7, #5284, Santa Cruz Biotechnology; mouse monoclonal antibody, dilution 1:1000 in 5% milk in TBST); anti-human BRAFV600E (VE1, #E19290, Spring Bioscience; mouse monoclonal antibody, dilution 1:400 in 1% milk in TBST); anti-pMEK (#9154, Cell Signaling; rabbit monoclonal antibody, dilution 1:1000 in 3% BSA in TBST); anti-α-TUBULIN (#T9026, Sigma-Aldrich; mouse monoclonal antibody, dilution 1:20000 in 5% milk in TBST); anti-EGFP (#A111-22, Molecular Probes, rabbit polyclonal antibody, dilution 1:2000 in 5% milk in TBST).

    Article Title: Investigation of FOXM1 as a Potential New Target for Melanoma
    Article Snippet: Sections of paraffin-embedded melanomas and nevus tissue samples were stained with a monoclonal mouse anti-FOXM1 antibody (clone 3A9; Abnova, Taipei, Taiwan), monoclonal mouse anti-BRAFV600E antibody (clone VE1; Spring Bioscience, Pleasanton, CA) and monoclonal rabbit anti-phospho-AKT (Ser473) antibody (Cell Signaling Technologies, Tokyo, Japan).

    Article Title: Sessile Serrated Polyps are Precursors of Colon Carcinomas Predominantly with Deficient DNA Mismatch Repair
    Article Snippet: Staining for BRAF was performed with a pan-BRAF antibody (pBR1 clone; Spring Bioscience, Inc., Pleasanton, CA) diluted 1:100 at 37°C for 16 min. We then used an anti- BRAFV600E mouse monoclonal antibody (VE1 clone; Spring Bioscience, Inc.) raised against an immunogenic synthetic peptide derived from the internal region of the BRAFV600E protein.

    Article Title: Immunohistochemistry as an accurate tool for evaluating BRAF-V600E mutation in 130 samples of papillary thyroid cancer.
    Article Snippet: Background.. BRAF mutation has been investigated by immunohistochemistry and has shown high sensitivity and specificity.. We aim to investigate the accuracy of immunohistochemistry versus molecular testing of BRAF in papillary thyroid cancer using a large number of polymerase chain reaction–positive BRAF papillary thyroid cancer tissues.

    Article Title: BRAF V600E immunopositive Melanomas Show Low Frequency of Heterogeneity and Association With Epithelioid Tumor Cells
    Article Snippet: In brief, antigen retrieval was performed with EDTA (pH 9) using the PT link (Dako) for 10 minutes at 97 °C, subsequently followed by 5 minutes blocking with EnVision FLEX Peroxidase-Blocking Reagent, 20-minutes primary antibody incubation with “Mouse Anti-Human BRAFV600E Monoclonal Antibody VE1 (E19292—Spring Bioscience [1:50 dilution]), 15 minutes incubation with EnVision FLEX + Mouse (Linker) and 20 minutes incubation with Envision FLEX HRP-labeled polymer.

    Colony Assay:

    Article Title: BRAFV600E immunopositive Melanomas Show Low Frequency of Heterogeneity and Association With Epithelioid Tumor Cells
    Article Snippet: In brief, antigen retrieval was performed with EDTA (pH 9) using the PT link (Dako) for 10 minutes at 97 8C, subsequently followed by 5 minutes blocking with EnVision FLEX Peroxidase-Blocking Reagent, 20-minutes primary antibody incubation with ‘‘Mouse Anti-Human BRAFV600E Monoclonal Antibody VE1 (E19292—Spring Bioscience [1:50 dilution]), 15 minutes incubation with EnVision FLEXþMouse (Linker) and 20 minutes incubation with Envision FLEX HRP-labeled polymer.

    Article Title: Clinicopathologic correlations of the BRAFV600E mutation, BRAF V600E immunohistochemistry, and BRAF RNA in situ hybridization in papillary thyroid carcinoma.
    Article Snippet: Background: The BRAFV600E mutation is the most common genetic alteration in papillary thyroid carcinoma (PTC).. The aim of this study is to analyze the clinicopathologic correlations of the BRAFV600E mutation, BRAF V600E immunohistochemistry (IHC) and BRAF RNA in situ hybridization (ISH) in PTC.. Methods: This study included 467 patients with PTC who underwent surgical resection.

    Article Title: Syringocystadenoma papilliferum associated with verrucous carcinoma of the skin in the same lesion: Report of four cases.
    Article Snippet: The association of syringocystadenoma papilliferum with verrucous carcinoma of the skin in the same lesion is a rare, but well-documented event.. Although human papilloma viruses have been proposed to have an etiologic role in the development of the verrucous proliferations associated with syringocystadenoma papilliferum, most of the immunohistochemical and molecular studies have failed to demonstrate the presence of their genomic material in these lesions.. We report a series of 4 cases of syringocystadenoma papilliferum associated with verrucous carcinoma in anogenital lesions.

    Article Title: The landscape of BRAF transcript and protein variants in human cancer
    Article Snippet: They were then incubated overnight at 4 °C with the following primary antibodies: anti-BRAF (F-7, #5284, Santa Cruz Biotechnology; mouse monoclonal antibody, dilution 1:1000 in 5% milk in TBST); anti-human BRAFV600E (VE1, #E19290, Spring Bioscience; mouse monoclonal antibody, dilution 1:400 in 1% milk in TBST); anti-pMEK (#9154, Cell Signaling; rabbit monoclonal antibody, dilution 1:1000 in 3% BSA in TBST); anti-α-TUBULIN (#T9026, Sigma-Aldrich; mouse monoclonal antibody, dilution 1:20000 in 5% milk in TBST); anti-EGFP (#A111-22, Molecular Probes, rabbit polyclonal antibody, dilution 1:2000 in 5% milk in TBST).

    Article Title: Investigation of FOXM1 as a Potential New Target for Melanoma
    Article Snippet: Sections of paraffin-embedded melanomas and nevus tissue samples were stained with a monoclonal mouse anti-FOXM1 antibody (clone 3A9; Abnova, Taipei, Taiwan), monoclonal mouse anti-BRAFV600E antibody (clone VE1; Spring Bioscience, Pleasanton, CA) and monoclonal rabbit anti-phospho-AKT (Ser473) antibody (Cell Signaling Technologies, Tokyo, Japan).

    Article Title: Sessile Serrated Polyps are Precursors of Colon Carcinomas Predominantly with Deficient DNA Mismatch Repair
    Article Snippet: Staining for BRAF was performed with a pan-BRAF antibody (pBR1 clone; Spring Bioscience, Inc., Pleasanton, CA) diluted 1:100 at 37°C for 16 min. We then used an anti- BRAFV600E mouse monoclonal antibody (VE1 clone; Spring Bioscience, Inc.) raised against an immunogenic synthetic peptide derived from the internal region of the BRAFV600E protein.

    Article Title: Immunohistochemistry as an accurate tool for evaluating BRAF-V600E mutation in 130 samples of papillary thyroid cancer.
    Article Snippet: Background.. BRAF mutation has been investigated by immunohistochemistry and has shown high sensitivity and specificity.. We aim to investigate the accuracy of immunohistochemistry versus molecular testing of BRAF in papillary thyroid cancer using a large number of polymerase chain reaction–positive BRAF papillary thyroid cancer tissues.

    Article Title: BRAF V600E immunopositive Melanomas Show Low Frequency of Heterogeneity and Association With Epithelioid Tumor Cells
    Article Snippet: In brief, antigen retrieval was performed with EDTA (pH 9) using the PT link (Dako) for 10 minutes at 97 °C, subsequently followed by 5 minutes blocking with EnVision FLEX Peroxidase-Blocking Reagent, 20-minutes primary antibody incubation with “Mouse Anti-Human BRAFV600E Monoclonal Antibody VE1 (E19292—Spring Bioscience [1:50 dilution]), 15 minutes incubation with EnVision FLEX + Mouse (Linker) and 20 minutes incubation with Envision FLEX HRP-labeled polymer.

    Immunoprecipitation:

    Article Title: BRAFV600E immunopositive Melanomas Show Low Frequency of Heterogeneity and Association With Epithelioid Tumor Cells
    Article Snippet: In brief, antigen retrieval was performed with EDTA (pH 9) using the PT link (Dako) for 10 minutes at 97 8C, subsequently followed by 5 minutes blocking with EnVision FLEX Peroxidase-Blocking Reagent, 20-minutes primary antibody incubation with ‘‘Mouse Anti-Human BRAFV600E Monoclonal Antibody VE1 (E19292—Spring Bioscience [1:50 dilution]), 15 minutes incubation with EnVision FLEXþMouse (Linker) and 20 minutes incubation with Envision FLEX HRP-labeled polymer.

    Article Title: Clinicopathologic correlations of the BRAFV600E mutation, BRAF V600E immunohistochemistry, and BRAF RNA in situ hybridization in papillary thyroid carcinoma.
    Article Snippet: Background: The BRAFV600E mutation is the most common genetic alteration in papillary thyroid carcinoma (PTC).. The aim of this study is to analyze the clinicopathologic correlations of the BRAFV600E mutation, BRAF V600E immunohistochemistry (IHC) and BRAF RNA in situ hybridization (ISH) in PTC.. Methods: This study included 467 patients with PTC who underwent surgical resection.

    Article Title: Syringocystadenoma papilliferum associated with verrucous carcinoma of the skin in the same lesion: Report of four cases.
    Article Snippet: The association of syringocystadenoma papilliferum with verrucous carcinoma of the skin in the same lesion is a rare, but well-documented event.. Although human papilloma viruses have been proposed to have an etiologic role in the development of the verrucous proliferations associated with syringocystadenoma papilliferum, most of the immunohistochemical and molecular studies have failed to demonstrate the presence of their genomic material in these lesions.. We report a series of 4 cases of syringocystadenoma papilliferum associated with verrucous carcinoma in anogenital lesions.

    Article Title: The landscape of BRAF transcript and protein variants in human cancer
    Article Snippet: They were then incubated overnight at 4 °C with the following primary antibodies: anti-BRAF (F-7, #5284, Santa Cruz Biotechnology; mouse monoclonal antibody, dilution 1:1000 in 5% milk in TBST); anti-human BRAFV600E (VE1, #E19290, Spring Bioscience; mouse monoclonal antibody, dilution 1:400 in 1% milk in TBST); anti-pMEK (#9154, Cell Signaling; rabbit monoclonal antibody, dilution 1:1000 in 3% BSA in TBST); anti-α-TUBULIN (#T9026, Sigma-Aldrich; mouse monoclonal antibody, dilution 1:20000 in 5% milk in TBST); anti-EGFP (#A111-22, Molecular Probes, rabbit polyclonal antibody, dilution 1:2000 in 5% milk in TBST).

    Article Title: Investigation of FOXM1 as a Potential New Target for Melanoma
    Article Snippet: Sections of paraffin-embedded melanomas and nevus tissue samples were stained with a monoclonal mouse anti-FOXM1 antibody (clone 3A9; Abnova, Taipei, Taiwan), monoclonal mouse anti-BRAFV600E antibody (clone VE1; Spring Bioscience, Pleasanton, CA) and monoclonal rabbit anti-phospho-AKT (Ser473) antibody (Cell Signaling Technologies, Tokyo, Japan).

    Article Title: Sessile Serrated Polyps are Precursors of Colon Carcinomas Predominantly with Deficient DNA Mismatch Repair
    Article Snippet: Staining for BRAF was performed with a pan-BRAF antibody (pBR1 clone; Spring Bioscience, Inc., Pleasanton, CA) diluted 1:100 at 37°C for 16 min. We then used an anti- BRAFV600E mouse monoclonal antibody (VE1 clone; Spring Bioscience, Inc.) raised against an immunogenic synthetic peptide derived from the internal region of the BRAFV600E protein.

    Article Title: Immunohistochemistry as an accurate tool for evaluating BRAF-V600E mutation in 130 samples of papillary thyroid cancer.
    Article Snippet: Background.. BRAF mutation has been investigated by immunohistochemistry and has shown high sensitivity and specificity.. We aim to investigate the accuracy of immunohistochemistry versus molecular testing of BRAF in papillary thyroid cancer using a large number of polymerase chain reaction–positive BRAF papillary thyroid cancer tissues.

    Article Title: BRAF V600E immunopositive Melanomas Show Low Frequency of Heterogeneity and Association With Epithelioid Tumor Cells
    Article Snippet: In brief, antigen retrieval was performed with EDTA (pH 9) using the PT link (Dako) for 10 minutes at 97 °C, subsequently followed by 5 minutes blocking with EnVision FLEX Peroxidase-Blocking Reagent, 20-minutes primary antibody incubation with “Mouse Anti-Human BRAFV600E Monoclonal Antibody VE1 (E19292—Spring Bioscience [1:50 dilution]), 15 minutes incubation with EnVision FLEX + Mouse (Linker) and 20 minutes incubation with Envision FLEX HRP-labeled polymer.

    Negative Control:

    Article Title: BRAFV600E immunopositive Melanomas Show Low Frequency of Heterogeneity and Association With Epithelioid Tumor Cells
    Article Snippet: In brief, antigen retrieval was performed with EDTA (pH 9) using the PT link (Dako) for 10 minutes at 97 8C, subsequently followed by 5 minutes blocking with EnVision FLEX Peroxidase-Blocking Reagent, 20-minutes primary antibody incubation with ‘‘Mouse Anti-Human BRAFV600E Monoclonal Antibody VE1 (E19292—Spring Bioscience [1:50 dilution]), 15 minutes incubation with EnVision FLEXþMouse (Linker) and 20 minutes incubation with Envision FLEX HRP-labeled polymer.

    Article Title: Clinicopathologic correlations of the BRAFV600E mutation, BRAF V600E immunohistochemistry, and BRAF RNA in situ hybridization in papillary thyroid carcinoma.
    Article Snippet: Background: The BRAFV600E mutation is the most common genetic alteration in papillary thyroid carcinoma (PTC).. The aim of this study is to analyze the clinicopathologic correlations of the BRAFV600E mutation, BRAF V600E immunohistochemistry (IHC) and BRAF RNA in situ hybridization (ISH) in PTC.. Methods: This study included 467 patients with PTC who underwent surgical resection.

    Article Title: Syringocystadenoma papilliferum associated with verrucous carcinoma of the skin in the same lesion: Report of four cases.
    Article Snippet: The association of syringocystadenoma papilliferum with verrucous carcinoma of the skin in the same lesion is a rare, but well-documented event.. Although human papilloma viruses have been proposed to have an etiologic role in the development of the verrucous proliferations associated with syringocystadenoma papilliferum, most of the immunohistochemical and molecular studies have failed to demonstrate the presence of their genomic material in these lesions.. We report a series of 4 cases of syringocystadenoma papilliferum associated with verrucous carcinoma in anogenital lesions.

    Article Title: The landscape of BRAF transcript and protein variants in human cancer
    Article Snippet: They were then incubated overnight at 4 °C with the following primary antibodies: anti-BRAF (F-7, #5284, Santa Cruz Biotechnology; mouse monoclonal antibody, dilution 1:1000 in 5% milk in TBST); anti-human BRAFV600E (VE1, #E19290, Spring Bioscience; mouse monoclonal antibody, dilution 1:400 in 1% milk in TBST); anti-pMEK (#9154, Cell Signaling; rabbit monoclonal antibody, dilution 1:1000 in 3% BSA in TBST); anti-α-TUBULIN (#T9026, Sigma-Aldrich; mouse monoclonal antibody, dilution 1:20000 in 5% milk in TBST); anti-EGFP (#A111-22, Molecular Probes, rabbit polyclonal antibody, dilution 1:2000 in 5% milk in TBST).

    Article Title: Investigation of FOXM1 as a Potential New Target for Melanoma
    Article Snippet: Sections of paraffin-embedded melanomas and nevus tissue samples were stained with a monoclonal mouse anti-FOXM1 antibody (clone 3A9; Abnova, Taipei, Taiwan), monoclonal mouse anti-BRAFV600E antibody (clone VE1; Spring Bioscience, Pleasanton, CA) and monoclonal rabbit anti-phospho-AKT (Ser473) antibody (Cell Signaling Technologies, Tokyo, Japan).

    Article Title: Sessile Serrated Polyps are Precursors of Colon Carcinomas Predominantly with Deficient DNA Mismatch Repair
    Article Snippet: Staining for BRAF was performed with a pan-BRAF antibody (pBR1 clone; Spring Bioscience, Inc., Pleasanton, CA) diluted 1:100 at 37°C for 16 min. We then used an anti- BRAFV600E mouse monoclonal antibody (VE1 clone; Spring Bioscience, Inc.) raised against an immunogenic synthetic peptide derived from the internal region of the BRAFV600E protein.

    Article Title: Immunohistochemistry as an accurate tool for evaluating BRAF-V600E mutation in 130 samples of papillary thyroid cancer.
    Article Snippet: Background.. BRAF mutation has been investigated by immunohistochemistry and has shown high sensitivity and specificity.. We aim to investigate the accuracy of immunohistochemistry versus molecular testing of BRAF in papillary thyroid cancer using a large number of polymerase chain reaction–positive BRAF papillary thyroid cancer tissues.

    Article Title: BRAF V600E immunopositive Melanomas Show Low Frequency of Heterogeneity and Association With Epithelioid Tumor Cells
    Article Snippet: In brief, antigen retrieval was performed with EDTA (pH 9) using the PT link (Dako) for 10 minutes at 97 °C, subsequently followed by 5 minutes blocking with EnVision FLEX Peroxidase-Blocking Reagent, 20-minutes primary antibody incubation with “Mouse Anti-Human BRAFV600E Monoclonal Antibody VE1 (E19292—Spring Bioscience [1:50 dilution]), 15 minutes incubation with EnVision FLEX + Mouse (Linker) and 20 minutes incubation with Envision FLEX HRP-labeled polymer.

    Mass Spectrometry:

    Article Title: BRAFV600E immunopositive Melanomas Show Low Frequency of Heterogeneity and Association With Epithelioid Tumor Cells
    Article Snippet: In brief, antigen retrieval was performed with EDTA (pH 9) using the PT link (Dako) for 10 minutes at 97 8C, subsequently followed by 5 minutes blocking with EnVision FLEX Peroxidase-Blocking Reagent, 20-minutes primary antibody incubation with ‘‘Mouse Anti-Human BRAFV600E Monoclonal Antibody VE1 (E19292—Spring Bioscience [1:50 dilution]), 15 minutes incubation with EnVision FLEXþMouse (Linker) and 20 minutes incubation with Envision FLEX HRP-labeled polymer.

    Article Title: Clinicopathologic correlations of the BRAFV600E mutation, BRAF V600E immunohistochemistry, and BRAF RNA in situ hybridization in papillary thyroid carcinoma.
    Article Snippet: Background: The BRAFV600E mutation is the most common genetic alteration in papillary thyroid carcinoma (PTC).. The aim of this study is to analyze the clinicopathologic correlations of the BRAFV600E mutation, BRAF V600E immunohistochemistry (IHC) and BRAF RNA in situ hybridization (ISH) in PTC.. Methods: This study included 467 patients with PTC who underwent surgical resection.

    Article Title: Syringocystadenoma papilliferum associated with verrucous carcinoma of the skin in the same lesion: Report of four cases.
    Article Snippet: The association of syringocystadenoma papilliferum with verrucous carcinoma of the skin in the same lesion is a rare, but well-documented event.. Although human papilloma viruses have been proposed to have an etiologic role in the development of the verrucous proliferations associated with syringocystadenoma papilliferum, most of the immunohistochemical and molecular studies have failed to demonstrate the presence of their genomic material in these lesions.. We report a series of 4 cases of syringocystadenoma papilliferum associated with verrucous carcinoma in anogenital lesions.

    Article Title: The landscape of BRAF transcript and protein variants in human cancer
    Article Snippet: They were then incubated overnight at 4 °C with the following primary antibodies: anti-BRAF (F-7, #5284, Santa Cruz Biotechnology; mouse monoclonal antibody, dilution 1:1000 in 5% milk in TBST); anti-human BRAFV600E (VE1, #E19290, Spring Bioscience; mouse monoclonal antibody, dilution 1:400 in 1% milk in TBST); anti-pMEK (#9154, Cell Signaling; rabbit monoclonal antibody, dilution 1:1000 in 3% BSA in TBST); anti-α-TUBULIN (#T9026, Sigma-Aldrich; mouse monoclonal antibody, dilution 1:20000 in 5% milk in TBST); anti-EGFP (#A111-22, Molecular Probes, rabbit polyclonal antibody, dilution 1:2000 in 5% milk in TBST).

    Article Title: Investigation of FOXM1 as a Potential New Target for Melanoma
    Article Snippet: Sections of paraffin-embedded melanomas and nevus tissue samples were stained with a monoclonal mouse anti-FOXM1 antibody (clone 3A9; Abnova, Taipei, Taiwan), monoclonal mouse anti-BRAFV600E antibody (clone VE1; Spring Bioscience, Pleasanton, CA) and monoclonal rabbit anti-phospho-AKT (Ser473) antibody (Cell Signaling Technologies, Tokyo, Japan).

    Article Title: Sessile Serrated Polyps are Precursors of Colon Carcinomas Predominantly with Deficient DNA Mismatch Repair
    Article Snippet: Staining for BRAF was performed with a pan-BRAF antibody (pBR1 clone; Spring Bioscience, Inc., Pleasanton, CA) diluted 1:100 at 37°C for 16 min. We then used an anti- BRAFV600E mouse monoclonal antibody (VE1 clone; Spring Bioscience, Inc.) raised against an immunogenic synthetic peptide derived from the internal region of the BRAFV600E protein.

    Article Title: Immunohistochemistry as an accurate tool for evaluating BRAF-V600E mutation in 130 samples of papillary thyroid cancer.
    Article Snippet: Background.. BRAF mutation has been investigated by immunohistochemistry and has shown high sensitivity and specificity.. We aim to investigate the accuracy of immunohistochemistry versus molecular testing of BRAF in papillary thyroid cancer using a large number of polymerase chain reaction–positive BRAF papillary thyroid cancer tissues.

    Article Title: BRAF V600E immunopositive Melanomas Show Low Frequency of Heterogeneity and Association With Epithelioid Tumor Cells
    Article Snippet: In brief, antigen retrieval was performed with EDTA (pH 9) using the PT link (Dako) for 10 minutes at 97 °C, subsequently followed by 5 minutes blocking with EnVision FLEX Peroxidase-Blocking Reagent, 20-minutes primary antibody incubation with “Mouse Anti-Human BRAFV600E Monoclonal Antibody VE1 (E19292—Spring Bioscience [1:50 dilution]), 15 minutes incubation with EnVision FLEX + Mouse (Linker) and 20 minutes incubation with Envision FLEX HRP-labeled polymer.

    Liquid Chromatography with Mass Spectroscopy:

    Article Title: BRAFV600E immunopositive Melanomas Show Low Frequency of Heterogeneity and Association With Epithelioid Tumor Cells
    Article Snippet: In brief, antigen retrieval was performed with EDTA (pH 9) using the PT link (Dako) for 10 minutes at 97 8C, subsequently followed by 5 minutes blocking with EnVision FLEX Peroxidase-Blocking Reagent, 20-minutes primary antibody incubation with ‘‘Mouse Anti-Human BRAFV600E Monoclonal Antibody VE1 (E19292—Spring Bioscience [1:50 dilution]), 15 minutes incubation with EnVision FLEXþMouse (Linker) and 20 minutes incubation with Envision FLEX HRP-labeled polymer.

    Article Title: Clinicopathologic correlations of the BRAFV600E mutation, BRAF V600E immunohistochemistry, and BRAF RNA in situ hybridization in papillary thyroid carcinoma.
    Article Snippet: Background: The BRAFV600E mutation is the most common genetic alteration in papillary thyroid carcinoma (PTC).. The aim of this study is to analyze the clinicopathologic correlations of the BRAFV600E mutation, BRAF V600E immunohistochemistry (IHC) and BRAF RNA in situ hybridization (ISH) in PTC.. Methods: This study included 467 patients with PTC who underwent surgical resection.

    Article Title: Syringocystadenoma papilliferum associated with verrucous carcinoma of the skin in the same lesion: Report of four cases.
    Article Snippet: The association of syringocystadenoma papilliferum with verrucous carcinoma of the skin in the same lesion is a rare, but well-documented event.. Although human papilloma viruses have been proposed to have an etiologic role in the development of the verrucous proliferations associated with syringocystadenoma papilliferum, most of the immunohistochemical and molecular studies have failed to demonstrate the presence of their genomic material in these lesions.. We report a series of 4 cases of syringocystadenoma papilliferum associated with verrucous carcinoma in anogenital lesions.

    Article Title: The landscape of BRAF transcript and protein variants in human cancer
    Article Snippet: They were then incubated overnight at 4 °C with the following primary antibodies: anti-BRAF (F-7, #5284, Santa Cruz Biotechnology; mouse monoclonal antibody, dilution 1:1000 in 5% milk in TBST); anti-human BRAFV600E (VE1, #E19290, Spring Bioscience; mouse monoclonal antibody, dilution 1:400 in 1% milk in TBST); anti-pMEK (#9154, Cell Signaling; rabbit monoclonal antibody, dilution 1:1000 in 3% BSA in TBST); anti-α-TUBULIN (#T9026, Sigma-Aldrich; mouse monoclonal antibody, dilution 1:20000 in 5% milk in TBST); anti-EGFP (#A111-22, Molecular Probes, rabbit polyclonal antibody, dilution 1:2000 in 5% milk in TBST).

    Article Title: Investigation of FOXM1 as a Potential New Target for Melanoma
    Article Snippet: Sections of paraffin-embedded melanomas and nevus tissue samples were stained with a monoclonal mouse anti-FOXM1 antibody (clone 3A9; Abnova, Taipei, Taiwan), monoclonal mouse anti-BRAFV600E antibody (clone VE1; Spring Bioscience, Pleasanton, CA) and monoclonal rabbit anti-phospho-AKT (Ser473) antibody (Cell Signaling Technologies, Tokyo, Japan).

    Article Title: Sessile Serrated Polyps are Precursors of Colon Carcinomas Predominantly with Deficient DNA Mismatch Repair
    Article Snippet: Staining for BRAF was performed with a pan-BRAF antibody (pBR1 clone; Spring Bioscience, Inc., Pleasanton, CA) diluted 1:100 at 37°C for 16 min. We then used an anti- BRAFV600E mouse monoclonal antibody (VE1 clone; Spring Bioscience, Inc.) raised against an immunogenic synthetic peptide derived from the internal region of the BRAFV600E protein.

    Article Title: Immunohistochemistry as an accurate tool for evaluating BRAF-V600E mutation in 130 samples of papillary thyroid cancer.
    Article Snippet: Background.. BRAF mutation has been investigated by immunohistochemistry and has shown high sensitivity and specificity.. We aim to investigate the accuracy of immunohistochemistry versus molecular testing of BRAF in papillary thyroid cancer using a large number of polymerase chain reaction–positive BRAF papillary thyroid cancer tissues.

    Article Title: BRAF V600E immunopositive Melanomas Show Low Frequency of Heterogeneity and Association With Epithelioid Tumor Cells
    Article Snippet: In brief, antigen retrieval was performed with EDTA (pH 9) using the PT link (Dako) for 10 minutes at 97 °C, subsequently followed by 5 minutes blocking with EnVision FLEX Peroxidase-Blocking Reagent, 20-minutes primary antibody incubation with “Mouse Anti-Human BRAFV600E Monoclonal Antibody VE1 (E19292—Spring Bioscience [1:50 dilution]), 15 minutes incubation with EnVision FLEX + Mouse (Linker) and 20 minutes incubation with Envision FLEX HRP-labeled polymer.

    Infection:

    Article Title: BRAFV600E immunopositive Melanomas Show Low Frequency of Heterogeneity and Association With Epithelioid Tumor Cells
    Article Snippet: In brief, antigen retrieval was performed with EDTA (pH 9) using the PT link (Dako) for 10 minutes at 97 8C, subsequently followed by 5 minutes blocking with EnVision FLEX Peroxidase-Blocking Reagent, 20-minutes primary antibody incubation with ‘‘Mouse Anti-Human BRAFV600E Monoclonal Antibody VE1 (E19292—Spring Bioscience [1:50 dilution]), 15 minutes incubation with EnVision FLEXþMouse (Linker) and 20 minutes incubation with Envision FLEX HRP-labeled polymer.

    Article Title: Clinicopathologic correlations of the BRAFV600E mutation, BRAF V600E immunohistochemistry, and BRAF RNA in situ hybridization in papillary thyroid carcinoma.
    Article Snippet: Background: The BRAFV600E mutation is the most common genetic alteration in papillary thyroid carcinoma (PTC).. The aim of this study is to analyze the clinicopathologic correlations of the BRAFV600E mutation, BRAF V600E immunohistochemistry (IHC) and BRAF RNA in situ hybridization (ISH) in PTC.. Methods: This study included 467 patients with PTC who underwent surgical resection.

    Article Title: Syringocystadenoma papilliferum associated with verrucous carcinoma of the skin in the same lesion: Report of four cases.
    Article Snippet: The association of syringocystadenoma papilliferum with verrucous carcinoma of the skin in the same lesion is a rare, but well-documented event.. Although human papilloma viruses have been proposed to have an etiologic role in the development of the verrucous proliferations associated with syringocystadenoma papilliferum, most of the immunohistochemical and molecular studies have failed to demonstrate the presence of their genomic material in these lesions.. We report a series of 4 cases of syringocystadenoma papilliferum associated with verrucous carcinoma in anogenital lesions.

    Article Title: The landscape of BRAF transcript and protein variants in human cancer
    Article Snippet: They were then incubated overnight at 4 °C with the following primary antibodies: anti-BRAF (F-7, #5284, Santa Cruz Biotechnology; mouse monoclonal antibody, dilution 1:1000 in 5% milk in TBST); anti-human BRAFV600E (VE1, #E19290, Spring Bioscience; mouse monoclonal antibody, dilution 1:400 in 1% milk in TBST); anti-pMEK (#9154, Cell Signaling; rabbit monoclonal antibody, dilution 1:1000 in 3% BSA in TBST); anti-α-TUBULIN (#T9026, Sigma-Aldrich; mouse monoclonal antibody, dilution 1:20000 in 5% milk in TBST); anti-EGFP (#A111-22, Molecular Probes, rabbit polyclonal antibody, dilution 1:2000 in 5% milk in TBST).

    Article Title: Investigation of FOXM1 as a Potential New Target for Melanoma
    Article Snippet: Sections of paraffin-embedded melanomas and nevus tissue samples were stained with a monoclonal mouse anti-FOXM1 antibody (clone 3A9; Abnova, Taipei, Taiwan), monoclonal mouse anti-BRAFV600E antibody (clone VE1; Spring Bioscience, Pleasanton, CA) and monoclonal rabbit anti-phospho-AKT (Ser473) antibody (Cell Signaling Technologies, Tokyo, Japan).

    Article Title: Sessile Serrated Polyps are Precursors of Colon Carcinomas Predominantly with Deficient DNA Mismatch Repair
    Article Snippet: Staining for BRAF was performed with a pan-BRAF antibody (pBR1 clone; Spring Bioscience, Inc., Pleasanton, CA) diluted 1:100 at 37°C for 16 min. We then used an anti- BRAFV600E mouse monoclonal antibody (VE1 clone; Spring Bioscience, Inc.) raised against an immunogenic synthetic peptide derived from the internal region of the BRAFV600E protein.

    Article Title: Immunohistochemistry as an accurate tool for evaluating BRAF-V600E mutation in 130 samples of papillary thyroid cancer.
    Article Snippet: Background.. BRAF mutation has been investigated by immunohistochemistry and has shown high sensitivity and specificity.. We aim to investigate the accuracy of immunohistochemistry versus molecular testing of BRAF in papillary thyroid cancer using a large number of polymerase chain reaction–positive BRAF papillary thyroid cancer tissues.

    Article Title: BRAF V600E immunopositive Melanomas Show Low Frequency of Heterogeneity and Association With Epithelioid Tumor Cells
    Article Snippet: In brief, antigen retrieval was performed with EDTA (pH 9) using the PT link (Dako) for 10 minutes at 97 °C, subsequently followed by 5 minutes blocking with EnVision FLEX Peroxidase-Blocking Reagent, 20-minutes primary antibody incubation with “Mouse Anti-Human BRAFV600E Monoclonal Antibody VE1 (E19292—Spring Bioscience [1:50 dilution]), 15 minutes incubation with EnVision FLEX + Mouse (Linker) and 20 minutes incubation with Envision FLEX HRP-labeled polymer.

    Sequencing:

    Article Title: BRAFV600E immunopositive Melanomas Show Low Frequency of Heterogeneity and Association With Epithelioid Tumor Cells
    Article Snippet: In brief, antigen retrieval was performed with EDTA (pH 9) using the PT link (Dako) for 10 minutes at 97 8C, subsequently followed by 5 minutes blocking with EnVision FLEX Peroxidase-Blocking Reagent, 20-minutes primary antibody incubation with ‘‘Mouse Anti-Human BRAFV600E Monoclonal Antibody VE1 (E19292—Spring Bioscience [1:50 dilution]), 15 minutes incubation with EnVision FLEXþMouse (Linker) and 20 minutes incubation with Envision FLEX HRP-labeled polymer.

    Article Title: Clinicopathologic correlations of the BRAFV600E mutation, BRAF V600E immunohistochemistry, and BRAF RNA in situ hybridization in papillary thyroid carcinoma.
    Article Snippet: Background: The BRAFV600E mutation is the most common genetic alteration in papillary thyroid carcinoma (PTC).. The aim of this study is to analyze the clinicopathologic correlations of the BRAFV600E mutation, BRAF V600E immunohistochemistry (IHC) and BRAF RNA in situ hybridization (ISH) in PTC.. Methods: This study included 467 patients with PTC who underwent surgical resection.

    Article Title: Syringocystadenoma papilliferum associated with verrucous carcinoma of the skin in the same lesion: Report of four cases.
    Article Snippet: The association of syringocystadenoma papilliferum with verrucous carcinoma of the skin in the same lesion is a rare, but well-documented event.. Although human papilloma viruses have been proposed to have an etiologic role in the development of the verrucous proliferations associated with syringocystadenoma papilliferum, most of the immunohistochemical and molecular studies have failed to demonstrate the presence of their genomic material in these lesions.. We report a series of 4 cases of syringocystadenoma papilliferum associated with verrucous carcinoma in anogenital lesions.

    Article Title: The landscape of BRAF transcript and protein variants in human cancer
    Article Snippet: They were then incubated overnight at 4 °C with the following primary antibodies: anti-BRAF (F-7, #5284, Santa Cruz Biotechnology; mouse monoclonal antibody, dilution 1:1000 in 5% milk in TBST); anti-human BRAFV600E (VE1, #E19290, Spring Bioscience; mouse monoclonal antibody, dilution 1:400 in 1% milk in TBST); anti-pMEK (#9154, Cell Signaling; rabbit monoclonal antibody, dilution 1:1000 in 3% BSA in TBST); anti-α-TUBULIN (#T9026, Sigma-Aldrich; mouse monoclonal antibody, dilution 1:20000 in 5% milk in TBST); anti-EGFP (#A111-22, Molecular Probes, rabbit polyclonal antibody, dilution 1:2000 in 5% milk in TBST).

    Article Title: Investigation of FOXM1 as a Potential New Target for Melanoma
    Article Snippet: Sections of paraffin-embedded melanomas and nevus tissue samples were stained with a monoclonal mouse anti-FOXM1 antibody (clone 3A9; Abnova, Taipei, Taiwan), monoclonal mouse anti-BRAFV600E antibody (clone VE1; Spring Bioscience, Pleasanton, CA) and monoclonal rabbit anti-phospho-AKT (Ser473) antibody (Cell Signaling Technologies, Tokyo, Japan).

    Article Title: Sessile Serrated Polyps are Precursors of Colon Carcinomas Predominantly with Deficient DNA Mismatch Repair
    Article Snippet: Staining for BRAF was performed with a pan-BRAF antibody (pBR1 clone; Spring Bioscience, Inc., Pleasanton, CA) diluted 1:100 at 37°C for 16 min. We then used an anti- BRAFV600E mouse monoclonal antibody (VE1 clone; Spring Bioscience, Inc.) raised against an immunogenic synthetic peptide derived from the internal region of the BRAFV600E protein.

    Article Title: Immunohistochemistry as an accurate tool for evaluating BRAF-V600E mutation in 130 samples of papillary thyroid cancer.
    Article Snippet: Background.. BRAF mutation has been investigated by immunohistochemistry and has shown high sensitivity and specificity.. We aim to investigate the accuracy of immunohistochemistry versus molecular testing of BRAF in papillary thyroid cancer using a large number of polymerase chain reaction–positive BRAF papillary thyroid cancer tissues.

    Article Title: BRAF V600E immunopositive Melanomas Show Low Frequency of Heterogeneity and Association With Epithelioid Tumor Cells
    Article Snippet: In brief, antigen retrieval was performed with EDTA (pH 9) using the PT link (Dako) for 10 minutes at 97 °C, subsequently followed by 5 minutes blocking with EnVision FLEX Peroxidase-Blocking Reagent, 20-minutes primary antibody incubation with “Mouse Anti-Human BRAFV600E Monoclonal Antibody VE1 (E19292—Spring Bioscience [1:50 dilution]), 15 minutes incubation with EnVision FLEX + Mouse (Linker) and 20 minutes incubation with Envision FLEX HRP-labeled polymer.

    Mutagenesis:

    Article Title: BRAFV600E immunopositive Melanomas Show Low Frequency of Heterogeneity and Association With Epithelioid Tumor Cells
    Article Snippet: In brief, antigen retrieval was performed with EDTA (pH 9) using the PT link (Dako) for 10 minutes at 97 8C, subsequently followed by 5 minutes blocking with EnVision FLEX Peroxidase-Blocking Reagent, 20-minutes primary antibody incubation with ‘‘Mouse Anti-Human BRAFV600E Monoclonal Antibody VE1 (E19292—Spring Bioscience [1:50 dilution]), 15 minutes incubation with EnVision FLEXþMouse (Linker) and 20 minutes incubation with Envision FLEX HRP-labeled polymer.

    Article Title: Clinicopathologic correlations of the BRAFV600E mutation, BRAF V600E immunohistochemistry, and BRAF RNA in situ hybridization in papillary thyroid carcinoma.
    Article Snippet: Background: The BRAFV600E mutation is the most common genetic alteration in papillary thyroid carcinoma (PTC).. The aim of this study is to analyze the clinicopathologic correlations of the BRAFV600E mutation, BRAF V600E immunohistochemistry (IHC) and BRAF RNA in situ hybridization (ISH) in PTC.. Methods: This study included 467 patients with PTC who underwent surgical resection.

    Article Title: Syringocystadenoma papilliferum associated with verrucous carcinoma of the skin in the same lesion: Report of four cases.
    Article Snippet: The association of syringocystadenoma papilliferum with verrucous carcinoma of the skin in the same lesion is a rare, but well-documented event.. Although human papilloma viruses have been proposed to have an etiologic role in the development of the verrucous proliferations associated with syringocystadenoma papilliferum, most of the immunohistochemical and molecular studies have failed to demonstrate the presence of their genomic material in these lesions.. We report a series of 4 cases of syringocystadenoma papilliferum associated with verrucous carcinoma in anogenital lesions.

    Article Title: The landscape of BRAF transcript and protein variants in human cancer
    Article Snippet: They were then incubated overnight at 4 °C with the following primary antibodies: anti-BRAF (F-7, #5284, Santa Cruz Biotechnology; mouse monoclonal antibody, dilution 1:1000 in 5% milk in TBST); anti-human BRAFV600E (VE1, #E19290, Spring Bioscience; mouse monoclonal antibody, dilution 1:400 in 1% milk in TBST); anti-pMEK (#9154, Cell Signaling; rabbit monoclonal antibody, dilution 1:1000 in 3% BSA in TBST); anti-α-TUBULIN (#T9026, Sigma-Aldrich; mouse monoclonal antibody, dilution 1:20000 in 5% milk in TBST); anti-EGFP (#A111-22, Molecular Probes, rabbit polyclonal antibody, dilution 1:2000 in 5% milk in TBST).

    Article Title: Investigation of FOXM1 as a Potential New Target for Melanoma
    Article Snippet: Sections of paraffin-embedded melanomas and nevus tissue samples were stained with a monoclonal mouse anti-FOXM1 antibody (clone 3A9; Abnova, Taipei, Taiwan), monoclonal mouse anti-BRAFV600E antibody (clone VE1; Spring Bioscience, Pleasanton, CA) and monoclonal rabbit anti-phospho-AKT (Ser473) antibody (Cell Signaling Technologies, Tokyo, Japan).

    Article Title: Sessile Serrated Polyps are Precursors of Colon Carcinomas Predominantly with Deficient DNA Mismatch Repair
    Article Snippet: Staining for BRAF was performed with a pan-BRAF antibody (pBR1 clone; Spring Bioscience, Inc., Pleasanton, CA) diluted 1:100 at 37°C for 16 min. We then used an anti- BRAFV600E mouse monoclonal antibody (VE1 clone; Spring Bioscience, Inc.) raised against an immunogenic synthetic peptide derived from the internal region of the BRAFV600E protein.

    Article Title: Immunohistochemistry as an accurate tool for evaluating BRAF-V600E mutation in 130 samples of papillary thyroid cancer.
    Article Snippet: Background.. BRAF mutation has been investigated by immunohistochemistry and has shown high sensitivity and specificity.. We aim to investigate the accuracy of immunohistochemistry versus molecular testing of BRAF in papillary thyroid cancer using a large number of polymerase chain reaction–positive BRAF papillary thyroid cancer tissues.

    Article Title: BRAF V600E immunopositive Melanomas Show Low Frequency of Heterogeneity and Association With Epithelioid Tumor Cells
    Article Snippet: In brief, antigen retrieval was performed with EDTA (pH 9) using the PT link (Dako) for 10 minutes at 97 °C, subsequently followed by 5 minutes blocking with EnVision FLEX Peroxidase-Blocking Reagent, 20-minutes primary antibody incubation with “Mouse Anti-Human BRAFV600E Monoclonal Antibody VE1 (E19292—Spring Bioscience [1:50 dilution]), 15 minutes incubation with EnVision FLEX + Mouse (Linker) and 20 minutes incubation with Envision FLEX HRP-labeled polymer.

    Flow Cytometry:

    Article Title: BRAFV600E immunopositive Melanomas Show Low Frequency of Heterogeneity and Association With Epithelioid Tumor Cells
    Article Snippet: In brief, antigen retrieval was performed with EDTA (pH 9) using the PT link (Dako) for 10 minutes at 97 8C, subsequently followed by 5 minutes blocking with EnVision FLEX Peroxidase-Blocking Reagent, 20-minutes primary antibody incubation with ‘‘Mouse Anti-Human BRAFV600E Monoclonal Antibody VE1 (E19292—Spring Bioscience [1:50 dilution]), 15 minutes incubation with EnVision FLEXþMouse (Linker) and 20 minutes incubation with Envision FLEX HRP-labeled polymer.

    Article Title: Clinicopathologic correlations of the BRAFV600E mutation, BRAF V600E immunohistochemistry, and BRAF RNA in situ hybridization in papillary thyroid carcinoma.
    Article Snippet: Background: The BRAFV600E mutation is the most common genetic alteration in papillary thyroid carcinoma (PTC).. The aim of this study is to analyze the clinicopathologic correlations of the BRAFV600E mutation, BRAF V600E immunohistochemistry (IHC) and BRAF RNA in situ hybridization (ISH) in PTC.. Methods: This study included 467 patients with PTC who underwent surgical resection.

    Article Title: Syringocystadenoma papilliferum associated with verrucous carcinoma of the skin in the same lesion: Report of four cases.
    Article Snippet: The association of syringocystadenoma papilliferum with verrucous carcinoma of the skin in the same lesion is a rare, but well-documented event.. Although human papilloma viruses have been proposed to have an etiologic role in the development of the verrucous proliferations associated with syringocystadenoma papilliferum, most of the immunohistochemical and molecular studies have failed to demonstrate the presence of their genomic material in these lesions.. We report a series of 4 cases of syringocystadenoma papilliferum associated with verrucous carcinoma in anogenital lesions.

    Article Title: The landscape of BRAF transcript and protein variants in human cancer
    Article Snippet: They were then incubated overnight at 4 °C with the following primary antibodies: anti-BRAF (F-7, #5284, Santa Cruz Biotechnology; mouse monoclonal antibody, dilution 1:1000 in 5% milk in TBST); anti-human BRAFV600E (VE1, #E19290, Spring Bioscience; mouse monoclonal antibody, dilution 1:400 in 1% milk in TBST); anti-pMEK (#9154, Cell Signaling; rabbit monoclonal antibody, dilution 1:1000 in 3% BSA in TBST); anti-α-TUBULIN (#T9026, Sigma-Aldrich; mouse monoclonal antibody, dilution 1:20000 in 5% milk in TBST); anti-EGFP (#A111-22, Molecular Probes, rabbit polyclonal antibody, dilution 1:2000 in 5% milk in TBST).

    Article Title: Investigation of FOXM1 as a Potential New Target for Melanoma
    Article Snippet: Sections of paraffin-embedded melanomas and nevus tissue samples were stained with a monoclonal mouse anti-FOXM1 antibody (clone 3A9; Abnova, Taipei, Taiwan), monoclonal mouse anti-BRAFV600E antibody (clone VE1; Spring Bioscience, Pleasanton, CA) and monoclonal rabbit anti-phospho-AKT (Ser473) antibody (Cell Signaling Technologies, Tokyo, Japan).

    Article Title: Sessile Serrated Polyps are Precursors of Colon Carcinomas Predominantly with Deficient DNA Mismatch Repair
    Article Snippet: Staining for BRAF was performed with a pan-BRAF antibody (pBR1 clone; Spring Bioscience, Inc., Pleasanton, CA) diluted 1:100 at 37°C for 16 min. We then used an anti- BRAFV600E mouse monoclonal antibody (VE1 clone; Spring Bioscience, Inc.) raised against an immunogenic synthetic peptide derived from the internal region of the BRAFV600E protein.

    Article Title: Immunohistochemistry as an accurate tool for evaluating BRAF-V600E mutation in 130 samples of papillary thyroid cancer.
    Article Snippet: Background.. BRAF mutation has been investigated by immunohistochemistry and has shown high sensitivity and specificity.. We aim to investigate the accuracy of immunohistochemistry versus molecular testing of BRAF in papillary thyroid cancer using a large number of polymerase chain reaction–positive BRAF papillary thyroid cancer tissues.

    Article Title: BRAF V600E immunopositive Melanomas Show Low Frequency of Heterogeneity and Association With Epithelioid Tumor Cells
    Article Snippet: In brief, antigen retrieval was performed with EDTA (pH 9) using the PT link (Dako) for 10 minutes at 97 °C, subsequently followed by 5 minutes blocking with EnVision FLEX Peroxidase-Blocking Reagent, 20-minutes primary antibody incubation with “Mouse Anti-Human BRAFV600E Monoclonal Antibody VE1 (E19292—Spring Bioscience [1:50 dilution]), 15 minutes incubation with EnVision FLEX + Mouse (Linker) and 20 minutes incubation with Envision FLEX HRP-labeled polymer.

    Confocal Microscopy:

    Article Title: BRAFV600E immunopositive Melanomas Show Low Frequency of Heterogeneity and Association With Epithelioid Tumor Cells
    Article Snippet: In brief, antigen retrieval was performed with EDTA (pH 9) using the PT link (Dako) for 10 minutes at 97 8C, subsequently followed by 5 minutes blocking with EnVision FLEX Peroxidase-Blocking Reagent, 20-minutes primary antibody incubation with ‘‘Mouse Anti-Human BRAFV600E Monoclonal Antibody VE1 (E19292—Spring Bioscience [1:50 dilution]), 15 minutes incubation with EnVision FLEXþMouse (Linker) and 20 minutes incubation with Envision FLEX HRP-labeled polymer.

    Article Title: Clinicopathologic correlations of the BRAFV600E mutation, BRAF V600E immunohistochemistry, and BRAF RNA in situ hybridization in papillary thyroid carcinoma.
    Article Snippet: Background: The BRAFV600E mutation is the most common genetic alteration in papillary thyroid carcinoma (PTC).. The aim of this study is to analyze the clinicopathologic correlations of the BRAFV600E mutation, BRAF V600E immunohistochemistry (IHC) and BRAF RNA in situ hybridization (ISH) in PTC.. Methods: This study included 467 patients with PTC who underwent surgical resection.

    Article Title: Syringocystadenoma papilliferum associated with verrucous carcinoma of the skin in the same lesion: Report of four cases.
    Article Snippet: The association of syringocystadenoma papilliferum with verrucous carcinoma of the skin in the same lesion is a rare, but well-documented event.. Although human papilloma viruses have been proposed to have an etiologic role in the development of the verrucous proliferations associated with syringocystadenoma papilliferum, most of the immunohistochemical and molecular studies have failed to demonstrate the presence of their genomic material in these lesions.. We report a series of 4 cases of syringocystadenoma papilliferum associated with verrucous carcinoma in anogenital lesions.

    Article Title: The landscape of BRAF transcript and protein variants in human cancer
    Article Snippet: They were then incubated overnight at 4 °C with the following primary antibodies: anti-BRAF (F-7, #5284, Santa Cruz Biotechnology; mouse monoclonal antibody, dilution 1:1000 in 5% milk in TBST); anti-human BRAFV600E (VE1, #E19290, Spring Bioscience; mouse monoclonal antibody, dilution 1:400 in 1% milk in TBST); anti-pMEK (#9154, Cell Signaling; rabbit monoclonal antibody, dilution 1:1000 in 3% BSA in TBST); anti-α-TUBULIN (#T9026, Sigma-Aldrich; mouse monoclonal antibody, dilution 1:20000 in 5% milk in TBST); anti-EGFP (#A111-22, Molecular Probes, rabbit polyclonal antibody, dilution 1:2000 in 5% milk in TBST).

    Article Title: Investigation of FOXM1 as a Potential New Target for Melanoma
    Article Snippet: Sections of paraffin-embedded melanomas and nevus tissue samples were stained with a monoclonal mouse anti-FOXM1 antibody (clone 3A9; Abnova, Taipei, Taiwan), monoclonal mouse anti-BRAFV600E antibody (clone VE1; Spring Bioscience, Pleasanton, CA) and monoclonal rabbit anti-phospho-AKT (Ser473) antibody (Cell Signaling Technologies, Tokyo, Japan).

    Article Title: Sessile Serrated Polyps are Precursors of Colon Carcinomas Predominantly with Deficient DNA Mismatch Repair
    Article Snippet: Staining for BRAF was performed with a pan-BRAF antibody (pBR1 clone; Spring Bioscience, Inc., Pleasanton, CA) diluted 1:100 at 37°C for 16 min. We then used an anti- BRAFV600E mouse monoclonal antibody (VE1 clone; Spring Bioscience, Inc.) raised against an immunogenic synthetic peptide derived from the internal region of the BRAFV600E protein.

    Article Title: Immunohistochemistry as an accurate tool for evaluating BRAF-V600E mutation in 130 samples of papillary thyroid cancer.
    Article Snippet: Background.. BRAF mutation has been investigated by immunohistochemistry and has shown high sensitivity and specificity.. We aim to investigate the accuracy of immunohistochemistry versus molecular testing of BRAF in papillary thyroid cancer using a large number of polymerase chain reaction–positive BRAF papillary thyroid cancer tissues.

    Article Title: BRAF V600E immunopositive Melanomas Show Low Frequency of Heterogeneity and Association With Epithelioid Tumor Cells
    Article Snippet: In brief, antigen retrieval was performed with EDTA (pH 9) using the PT link (Dako) for 10 minutes at 97 °C, subsequently followed by 5 minutes blocking with EnVision FLEX Peroxidase-Blocking Reagent, 20-minutes primary antibody incubation with “Mouse Anti-Human BRAFV600E Monoclonal Antibody VE1 (E19292—Spring Bioscience [1:50 dilution]), 15 minutes incubation with EnVision FLEX + Mouse (Linker) and 20 minutes incubation with Envision FLEX HRP-labeled polymer.

    Blocking Assay:

    Article Title: BRAFV600E immunopositive Melanomas Show Low Frequency of Heterogeneity and Association With Epithelioid Tumor Cells
    Article Snippet: In brief, antigen retrieval was performed with EDTA (pH 9) using the PT link (Dako) for 10 minutes at 97 8C, subsequently followed by 5 minutes blocking with EnVision FLEX Peroxidase-Blocking Reagent, 20-minutes primary antibody incubation with ‘‘Mouse Anti-Human BRAFV600E Monoclonal Antibody VE1 (E19292—Spring Bioscience [1:50 dilution]), 15 minutes incubation with EnVision FLEXþMouse (Linker) and 20 minutes incubation with Envision FLEX HRP-labeled polymer.

    Article Title: Clinicopathologic correlations of the BRAFV600E mutation, BRAF V600E immunohistochemistry, and BRAF RNA in situ hybridization in papillary thyroid carcinoma.
    Article Snippet: Background: The BRAFV600E mutation is the most common genetic alteration in papillary thyroid carcinoma (PTC).. The aim of this study is to analyze the clinicopathologic correlations of the BRAFV600E mutation, BRAF V600E immunohistochemistry (IHC) and BRAF RNA in situ hybridization (ISH) in PTC.. Methods: This study included 467 patients with PTC who underwent surgical resection.

    Article Title: Syringocystadenoma papilliferum associated with verrucous carcinoma of the skin in the same lesion: Report of four cases.
    Article Snippet: The association of syringocystadenoma papilliferum with verrucous carcinoma of the skin in the same lesion is a rare, but well-documented event.. Although human papilloma viruses have been proposed to have an etiologic role in the development of the verrucous proliferations associated with syringocystadenoma papilliferum, most of the immunohistochemical and molecular studies have failed to demonstrate the presence of their genomic material in these lesions.. We report a series of 4 cases of syringocystadenoma papilliferum associated with verrucous carcinoma in anogenital lesions.

    Article Title: The landscape of BRAF transcript and protein variants in human cancer
    Article Snippet: They were then incubated overnight at 4 °C with the following primary antibodies: anti-BRAF (F-7, #5284, Santa Cruz Biotechnology; mouse monoclonal antibody, dilution 1:1000 in 5% milk in TBST); anti-human BRAFV600E (VE1, #E19290, Spring Bioscience; mouse monoclonal antibody, dilution 1:400 in 1% milk in TBST); anti-pMEK (#9154, Cell Signaling; rabbit monoclonal antibody, dilution 1:1000 in 3% BSA in TBST); anti-α-TUBULIN (#T9026, Sigma-Aldrich; mouse monoclonal antibody, dilution 1:20000 in 5% milk in TBST); anti-EGFP (#A111-22, Molecular Probes, rabbit polyclonal antibody, dilution 1:2000 in 5% milk in TBST).

    Article Title: Investigation of FOXM1 as a Potential New Target for Melanoma
    Article Snippet: Sections of paraffin-embedded melanomas and nevus tissue samples were stained with a monoclonal mouse anti-FOXM1 antibody (clone 3A9; Abnova, Taipei, Taiwan), monoclonal mouse anti-BRAFV600E antibody (clone VE1; Spring Bioscience, Pleasanton, CA) and monoclonal rabbit anti-phospho-AKT (Ser473) antibody (Cell Signaling Technologies, Tokyo, Japan).

    Article Title: Sessile Serrated Polyps are Precursors of Colon Carcinomas Predominantly with Deficient DNA Mismatch Repair
    Article Snippet: Staining for BRAF was performed with a pan-BRAF antibody (pBR1 clone; Spring Bioscience, Inc., Pleasanton, CA) diluted 1:100 at 37°C for 16 min. We then used an anti- BRAFV600E mouse monoclonal antibody (VE1 clone; Spring Bioscience, Inc.) raised against an immunogenic synthetic peptide derived from the internal region of the BRAFV600E protein.

    Article Title: Immunohistochemistry as an accurate tool for evaluating BRAF-V600E mutation in 130 samples of papillary thyroid cancer.
    Article Snippet: Background.. BRAF mutation has been investigated by immunohistochemistry and has shown high sensitivity and specificity.. We aim to investigate the accuracy of immunohistochemistry versus molecular testing of BRAF in papillary thyroid cancer using a large number of polymerase chain reaction–positive BRAF papillary thyroid cancer tissues.

    Article Title: BRAF V600E immunopositive Melanomas Show Low Frequency of Heterogeneity and Association With Epithelioid Tumor Cells
    Article Snippet: In brief, antigen retrieval was performed with EDTA (pH 9) using the PT link (Dako) for 10 minutes at 97 °C, subsequently followed by 5 minutes blocking with EnVision FLEX Peroxidase-Blocking Reagent, 20-minutes primary antibody incubation with “Mouse Anti-Human BRAFV600E Monoclonal Antibody VE1 (E19292—Spring Bioscience [1:50 dilution]), 15 minutes incubation with EnVision FLEX + Mouse (Linker) and 20 minutes incubation with Envision FLEX HRP-labeled polymer.

    Polymer:

    Article Title: BRAFV600E immunopositive Melanomas Show Low Frequency of Heterogeneity and Association With Epithelioid Tumor Cells
    Article Snippet: In brief, antigen retrieval was performed with EDTA (pH 9) using the PT link (Dako) for 10 minutes at 97 8C, subsequently followed by 5 minutes blocking with EnVision FLEX Peroxidase-Blocking Reagent, 20-minutes primary antibody incubation with ‘‘Mouse Anti-Human BRAFV600E Monoclonal Antibody VE1 (E19292—Spring Bioscience [1:50 dilution]), 15 minutes incubation with EnVision FLEXþMouse (Linker) and 20 minutes incubation with Envision FLEX HRP-labeled polymer.

    Article Title: Clinicopathologic correlations of the BRAFV600E mutation, BRAF V600E immunohistochemistry, and BRAF RNA in situ hybridization in papillary thyroid carcinoma.
    Article Snippet: Background: The BRAFV600E mutation is the most common genetic alteration in papillary thyroid carcinoma (PTC).. The aim of this study is to analyze the clinicopathologic correlations of the BRAFV600E mutation, BRAF V600E immunohistochemistry (IHC) and BRAF RNA in situ hybridization (ISH) in PTC.. Methods: This study included 467 patients with PTC who underwent surgical resection.

    Article Title: Syringocystadenoma papilliferum associated with verrucous carcinoma of the skin in the same lesion: Report of four cases.
    Article Snippet: The association of syringocystadenoma papilliferum with verrucous carcinoma of the skin in the same lesion is a rare, but well-documented event.. Although human papilloma viruses have been proposed to have an etiologic role in the development of the verrucous proliferations associated with syringocystadenoma papilliferum, most of the immunohistochemical and molecular studies have failed to demonstrate the presence of their genomic material in these lesions.. We report a series of 4 cases of syringocystadenoma papilliferum associated with verrucous carcinoma in anogenital lesions.

    Article Title: The landscape of BRAF transcript and protein variants in human cancer
    Article Snippet: They were then incubated overnight at 4 °C with the following primary antibodies: anti-BRAF (F-7, #5284, Santa Cruz Biotechnology; mouse monoclonal antibody, dilution 1:1000 in 5% milk in TBST); anti-human BRAFV600E (VE1, #E19290, Spring Bioscience; mouse monoclonal antibody, dilution 1:400 in 1% milk in TBST); anti-pMEK (#9154, Cell Signaling; rabbit monoclonal antibody, dilution 1:1000 in 3% BSA in TBST); anti-α-TUBULIN (#T9026, Sigma-Aldrich; mouse monoclonal antibody, dilution 1:20000 in 5% milk in TBST); anti-EGFP (#A111-22, Molecular Probes, rabbit polyclonal antibody, dilution 1:2000 in 5% milk in TBST).

    Article Title: Investigation of FOXM1 as a Potential New Target for Melanoma
    Article Snippet: Sections of paraffin-embedded melanomas and nevus tissue samples were stained with a monoclonal mouse anti-FOXM1 antibody (clone 3A9; Abnova, Taipei, Taiwan), monoclonal mouse anti-BRAFV600E antibody (clone VE1; Spring Bioscience, Pleasanton, CA) and monoclonal rabbit anti-phospho-AKT (Ser473) antibody (Cell Signaling Technologies, Tokyo, Japan).

    Article Title: Sessile Serrated Polyps are Precursors of Colon Carcinomas Predominantly with Deficient DNA Mismatch Repair
    Article Snippet: Staining for BRAF was performed with a pan-BRAF antibody (pBR1 clone; Spring Bioscience, Inc., Pleasanton, CA) diluted 1:100 at 37°C for 16 min. We then used an anti- BRAFV600E mouse monoclonal antibody (VE1 clone; Spring Bioscience, Inc.) raised against an immunogenic synthetic peptide derived from the internal region of the BRAFV600E protein.

    Article Title: Immunohistochemistry as an accurate tool for evaluating BRAF-V600E mutation in 130 samples of papillary thyroid cancer.
    Article Snippet: Background.. BRAF mutation has been investigated by immunohistochemistry and has shown high sensitivity and specificity.. We aim to investigate the accuracy of immunohistochemistry versus molecular testing of BRAF in papillary thyroid cancer using a large number of polymerase chain reaction–positive BRAF papillary thyroid cancer tissues.

    Article Title: BRAF V600E immunopositive Melanomas Show Low Frequency of Heterogeneity and Association With Epithelioid Tumor Cells
    Article Snippet: In brief, antigen retrieval was performed with EDTA (pH 9) using the PT link (Dako) for 10 minutes at 97 °C, subsequently followed by 5 minutes blocking with EnVision FLEX Peroxidase-Blocking Reagent, 20-minutes primary antibody incubation with “Mouse Anti-Human BRAFV600E Monoclonal Antibody VE1 (E19292—Spring Bioscience [1:50 dilution]), 15 minutes incubation with EnVision FLEX + Mouse (Linker) and 20 minutes incubation with Envision FLEX HRP-labeled polymer.



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    The results of the immunohistochemical analysis of FOXM1,  BRAFV600E  and p-AKT.

    Journal: PLoS ONE

    Article Title: Investigation of FOXM1 as a Potential New Target for Melanoma

    doi: 10.1371/journal.pone.0144241

    Figure Lengend Snippet: The results of the immunohistochemical analysis of FOXM1, BRAFV600E and p-AKT.

    Article Snippet: Sections of paraffin-embedded melanomas and nevus tissue samples were stained with a monoclonal mouse anti-FOXM1 antibody (clone 3A9; Abnova, Taipei, Taiwan), monoclonal mouse anti-BRAFV600E antibody (clone VE1; Spring Bioscience, Pleasanton, CA) and monoclonal rabbit anti-phospho-AKT (Ser473) antibody (Cell Signaling Technologies, Tokyo, Japan).

    Techniques: Immunohistochemical staining